Heterologous expression of particulate methane monooxygenase in different host cells

Bing Han, Tao Su, Cheng Yang, Hao Jiang, Hao Wu, Chong Zhang, Xin Li, Xinhui Xing*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Methanotrophs use methane as the sole carbon and energy source, which cause slow growth, low cell density and hinder its industrial applications. One promising solution is to heterologously express methane monooxygenase (MMO) in other host cells that can be easily cultivated at high cell density. We systematically exploited the possibility of functional expression of pMMO by choosing different promoters and different host cells. The results showed that the recombinants could oxidize methane to methanol. In particular, ethanol could also be detected in the oxidized products, but the enzyme activity was instable, implying that some changes of pMMO expressed in the host cells might have occurred. In addition, SDS-PAGE analysis showed that many recombinants could express the subunits of pMMO, but the enzyme activity could not be detected. In conclusion, correct fold of pMMO in the host cells is important for its functional expression.

Original languageEnglish
Pages (from-to)1151-1159
Number of pages9
JournalShengwu Gongcheng Xuebao/Chinese Journal of Biotechnology
Volume25
Issue number8
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Biocatalysis
  • Heterologous expression
  • Methanotroph
  • Particulate methane monooxygenase (pMMO)

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Han, B., Su, T., Yang, C., Jiang, H., Wu, H., Zhang, C., Li, X., & Xing, X. (2009). Heterologous expression of particulate methane monooxygenase in different host cells. Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology, 25(8), 1151-1159.