Bai, H., Pan, Y., Tong, W., Zhang, W., Ren, X., Tian, F., Peng, B., Wang, X., Zhang, Y., Deng, Y., Qin, W., & Qian, X. (2013). Graphene based soft nanoreactors for facile "one-step" glycan enrichment and derivatization for MALDI-TOF-MS analysis. Talanta, 117, 1-7. https://doi.org/10.1016/j.talanta.2013.08.031
Bai, Haihong ; Pan, Yiting ; Tong, Wei et al. / Graphene based soft nanoreactors for facile "one-step" glycan enrichment and derivatization for MALDI-TOF-MS analysis. In: Talanta. 2013 ; Vol. 117. pp. 1-7.
@article{e03dbc466f354400886648eed4dd06b1,
title = "Graphene based soft nanoreactors for facile {"}one-step{"} glycan enrichment and derivatization for MALDI-TOF-MS analysis",
abstract = "Protein glycosylation is involved in the control of many important biological processes and structural alterations of the N-linked glycans are correlated with various kinds of disease. High-throughput N-glycan profiling is a key technique for elucidating the functions of glycans in biological process and disease development as well as discovering new diagnostic biomarkers. However, the low abundance of glycans existing in living organism, the competition/suppression effect of other highly abundant biological molecules and the inherent lack of alkalinity and hydrophobicity of glycans leads to particularly poor detection sensitivity in MS analysis. Here, we demonstrated the first {"}one-step{"} approach for highly efficient glycan enrichment and derivatization using reduced graphene oxide as nanoreactors and 1-pyrenebutyric hydrazide for glycan capture and derivatization, which resulted in a 33-fold increase in the glycan detection sensitivity in MALDI-TOF-MS and the identification of 48N-glycoforms from human plasma.",
keywords = "1-Pyrenebutyric hydrazide, Derivatization, Desalting, Enrichment, Glycan, Reduced graphene oxide",
author = "Haihong Bai and Yiting Pan and Wei Tong and Wanjun Zhang and Xiaojun Ren and Fang Tian and Bo Peng and Xin Wang and Yangjun Zhang and Yulin Deng and Weijie Qin and Xiaohong Qian",
year = "2013",
doi = "10.1016/j.talanta.2013.08.031",
language = "English",
volume = "117",
pages = "1--7",
journal = "Talanta",
issn = "0039-9140",
publisher = "Elsevier B.V.",
}
Bai, H, Pan, Y, Tong, W, Zhang, W, Ren, X, Tian, F, Peng, B, Wang, X, Zhang, Y, Deng, Y, Qin, W & Qian, X 2013, 'Graphene based soft nanoreactors for facile "one-step" glycan enrichment and derivatization for MALDI-TOF-MS analysis', Talanta, vol. 117, pp. 1-7. https://doi.org/10.1016/j.talanta.2013.08.031
Graphene based soft nanoreactors for facile "one-step" glycan enrichment and derivatization for MALDI-TOF-MS analysis. / Bai, Haihong; Pan, Yiting; Tong, Wei et al.
In:
Talanta, Vol. 117, 2013, p. 1-7.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Graphene based soft nanoreactors for facile "one-step" glycan enrichment and derivatization for MALDI-TOF-MS analysis
AU - Bai, Haihong
AU - Pan, Yiting
AU - Tong, Wei
AU - Zhang, Wanjun
AU - Ren, Xiaojun
AU - Tian, Fang
AU - Peng, Bo
AU - Wang, Xin
AU - Zhang, Yangjun
AU - Deng, Yulin
AU - Qin, Weijie
AU - Qian, Xiaohong
PY - 2013
Y1 - 2013
N2 - Protein glycosylation is involved in the control of many important biological processes and structural alterations of the N-linked glycans are correlated with various kinds of disease. High-throughput N-glycan profiling is a key technique for elucidating the functions of glycans in biological process and disease development as well as discovering new diagnostic biomarkers. However, the low abundance of glycans existing in living organism, the competition/suppression effect of other highly abundant biological molecules and the inherent lack of alkalinity and hydrophobicity of glycans leads to particularly poor detection sensitivity in MS analysis. Here, we demonstrated the first "one-step" approach for highly efficient glycan enrichment and derivatization using reduced graphene oxide as nanoreactors and 1-pyrenebutyric hydrazide for glycan capture and derivatization, which resulted in a 33-fold increase in the glycan detection sensitivity in MALDI-TOF-MS and the identification of 48N-glycoforms from human plasma.
AB - Protein glycosylation is involved in the control of many important biological processes and structural alterations of the N-linked glycans are correlated with various kinds of disease. High-throughput N-glycan profiling is a key technique for elucidating the functions of glycans in biological process and disease development as well as discovering new diagnostic biomarkers. However, the low abundance of glycans existing in living organism, the competition/suppression effect of other highly abundant biological molecules and the inherent lack of alkalinity and hydrophobicity of glycans leads to particularly poor detection sensitivity in MS analysis. Here, we demonstrated the first "one-step" approach for highly efficient glycan enrichment and derivatization using reduced graphene oxide as nanoreactors and 1-pyrenebutyric hydrazide for glycan capture and derivatization, which resulted in a 33-fold increase in the glycan detection sensitivity in MALDI-TOF-MS and the identification of 48N-glycoforms from human plasma.
KW - 1-Pyrenebutyric hydrazide
KW - Derivatization
KW - Desalting
KW - Enrichment
KW - Glycan
KW - Reduced graphene oxide
UR - http://www.scopus.com/inward/record.url?scp=84884335958&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2013.08.031
DO - 10.1016/j.talanta.2013.08.031
M3 - Article
C2 - 24209301
AN - SCOPUS:84884335958
SN - 0039-9140
VL - 117
SP - 1
EP - 7
JO - Talanta
JF - Talanta
ER -
Bai H, Pan Y, Tong W, Zhang W, Ren X, Tian F et al. Graphene based soft nanoreactors for facile "one-step" glycan enrichment and derivatization for MALDI-TOF-MS analysis. Talanta. 2013;117:1-7. doi: 10.1016/j.talanta.2013.08.031