Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9

Fang Yuan, Dongsheng Guo, Yanli Liu, Yingying Xu, Ge Gao, Yuhang Wu, Fan Yang, Xinrong Ke, Keyu Lai, Hongcheng Wei*, Yin Xiong Li

*Corresponding author for this work

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1 Citation (Scopus)

Abstract

The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.

Original languageEnglish
Pages (from-to)67-71
Number of pages5
JournalStem Cell Research
Volume26
DOIs
Publication statusPublished - Jan 2018
Externally publishedYes

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Yuan, F., Guo, D., Liu, Y., Xu, Y., Gao, G., Wu, Y., Yang, F., Ke, X., Lai, K., Wei, H., & Li, Y. X. (2018). Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9. Stem Cell Research, 26, 67-71. https://doi.org/10.1016/j.scr.2017.11.007