Yuan, F., Guo, D., Liu, Y., Xu, Y., Gao, G., Wu, Y., Yang, F., Ke, X., Lai, K., Wei, H., & Li, Y. X. (2018). Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9. Stem Cell Research, 26, 67-71. https://doi.org/10.1016/j.scr.2017.11.007
Yuan, Fang ; Guo, Dongsheng ; Liu, Yanli et al. / Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9. In: Stem Cell Research. 2018 ; Vol. 26. pp. 67-71.
@article{b02754535363444f95ddf6a8cb2cce61,
title = "Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9",
abstract = "The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.",
author = "Fang Yuan and Dongsheng Guo and Yanli Liu and Yingying Xu and Ge Gao and Yuhang Wu and Fan Yang and Xinrong Ke and Keyu Lai and Hongcheng Wei and Li, {Yin Xiong}",
note = "Publisher Copyright: {\textcopyright} 2017",
year = "2018",
month = jan,
doi = "10.1016/j.scr.2017.11.007",
language = "English",
volume = "26",
pages = "67--71",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier B.V.",
}
Yuan, F, Guo, D, Liu, Y, Xu, Y, Gao, G, Wu, Y, Yang, F, Ke, X, Lai, K, Wei, H & Li, YX 2018, 'Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9', Stem Cell Research, vol. 26, pp. 67-71. https://doi.org/10.1016/j.scr.2017.11.007
Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9. / Yuan, Fang; Guo, Dongsheng; Liu, Yanli et al.
In:
Stem Cell Research, Vol. 26, 01.2018, p. 67-71.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9
AU - Yuan, Fang
AU - Guo, Dongsheng
AU - Liu, Yanli
AU - Xu, Yingying
AU - Gao, Ge
AU - Wu, Yuhang
AU - Yang, Fan
AU - Ke, Xinrong
AU - Lai, Keyu
AU - Wei, Hongcheng
AU - Li, Yin Xiong
N1 - Publisher Copyright:
© 2017
PY - 2018/1
Y1 - 2018/1
N2 - The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.
AB - The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype.
UR - http://www.scopus.com/inward/record.url?scp=85037986258&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2017.11.007
DO - 10.1016/j.scr.2017.11.007
M3 - Article
C2 - 29247816
AN - SCOPUS:85037986258
SN - 1873-5061
VL - 26
SP - 67
EP - 71
JO - Stem Cell Research
JF - Stem Cell Research
ER -
Yuan F, Guo D, Liu Y, Xu Y, Gao G, Wu Y et al. Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9. Stem Cell Research. 2018 Jan;26:67-71. doi: 10.1016/j.scr.2017.11.007