Wu, F., Gao, G., Pan, T., Yang, Z., Xu, G., Abbas, N., Liu, Y., Chen, Y., Tan, S., You, K., Ke, X., Zhuang, Y., Lin, X., Yang, F., & Li, Y. X. (2018). Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing. Stem Cell Research, 27, 5-9. https://doi.org/10.1016/j.scr.2017.12.002
Wu, Feima ; Gao, Ge ; Pan, Tingcai et al. / Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing. In: Stem Cell Research. 2018 ; Vol. 27. pp. 5-9.
@article{7d7b67e5a367431580756ff2f532b5e2,
title = "Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing",
abstract = "The human SMO protein encoded by the smoothened (SMO) gene acts as a positive mediator for Hedgehog signaling. This pathway regulates many cellular activities, developmental morphogenesis, and tumorigenesis. Using CRISPR/Cas9 to edit human embryonic stem cell line WA01 (H1), we established a SMO mutant cell line (WAe001-A-16). This cell line has a 40 bp homozygous deletion in exon 2 of SMO leading to a shift in the open reading frame and early termination at amino acid position 287. WAe001-A-16 maintains a normal karyotype, parental cell morphology, pluripotency markers, and the capacity to differentiate into all three germline layers.",
author = "Feima Wu and Ge Gao and Tingcai Pan and Zhen Yang and Guosheng Xu and Nasir Abbas and Yanli Liu and Yan Chen and Shenglin Tan and Kai You and Xinrong Ke and Yuanqi Zhuang and Xianhua Lin and Fan Yang and Li, {Yin xiong}",
note = "Publisher Copyright: {\textcopyright} 2017 The Authors",
year = "2018",
month = mar,
doi = "10.1016/j.scr.2017.12.002",
language = "English",
volume = "27",
pages = "5--9",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier B.V.",
}
Wu, F, Gao, G, Pan, T, Yang, Z, Xu, G, Abbas, N, Liu, Y, Chen, Y, Tan, S, You, K, Ke, X, Zhuang, Y, Lin, X, Yang, F & Li, YX 2018, 'Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing', Stem Cell Research, vol. 27, pp. 5-9. https://doi.org/10.1016/j.scr.2017.12.002
Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing. / Wu, Feima
; Gao, Ge; Pan, Tingcai et al.
In:
Stem Cell Research, Vol. 27, 03.2018, p. 5-9.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing
AU - Wu, Feima
AU - Gao, Ge
AU - Pan, Tingcai
AU - Yang, Zhen
AU - Xu, Guosheng
AU - Abbas, Nasir
AU - Liu, Yanli
AU - Chen, Yan
AU - Tan, Shenglin
AU - You, Kai
AU - Ke, Xinrong
AU - Zhuang, Yuanqi
AU - Lin, Xianhua
AU - Yang, Fan
AU - Li, Yin xiong
N1 - Publisher Copyright:
© 2017 The Authors
PY - 2018/3
Y1 - 2018/3
N2 - The human SMO protein encoded by the smoothened (SMO) gene acts as a positive mediator for Hedgehog signaling. This pathway regulates many cellular activities, developmental morphogenesis, and tumorigenesis. Using CRISPR/Cas9 to edit human embryonic stem cell line WA01 (H1), we established a SMO mutant cell line (WAe001-A-16). This cell line has a 40 bp homozygous deletion in exon 2 of SMO leading to a shift in the open reading frame and early termination at amino acid position 287. WAe001-A-16 maintains a normal karyotype, parental cell morphology, pluripotency markers, and the capacity to differentiate into all three germline layers.
AB - The human SMO protein encoded by the smoothened (SMO) gene acts as a positive mediator for Hedgehog signaling. This pathway regulates many cellular activities, developmental morphogenesis, and tumorigenesis. Using CRISPR/Cas9 to edit human embryonic stem cell line WA01 (H1), we established a SMO mutant cell line (WAe001-A-16). This cell line has a 40 bp homozygous deletion in exon 2 of SMO leading to a shift in the open reading frame and early termination at amino acid position 287. WAe001-A-16 maintains a normal karyotype, parental cell morphology, pluripotency markers, and the capacity to differentiate into all three germline layers.
UR - http://www.scopus.com/inward/record.url?scp=85039718082&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2017.12.002
DO - 10.1016/j.scr.2017.12.002
M3 - Article
C2 - 29278761
AN - SCOPUS:85039718082
SN - 1873-5061
VL - 27
SP - 5
EP - 9
JO - Stem Cell Research
JF - Stem Cell Research
ER -
Wu F, Gao G, Pan T, Yang Z, Xu G, Abbas N et al. Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing. Stem Cell Research. 2018 Mar;27:5-9. doi: 10.1016/j.scr.2017.12.002