Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing

Feima Wu, Ge Gao, Tingcai Pan, Zhen Yang, Guosheng Xu, Nasir Abbas, Yanli Liu, Yan Chen, Shenglin Tan, Kai You, Xinrong Ke, Yuanqi Zhuang, Xianhua Lin, Fan Yang, Yin xiong Li*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The human SMO protein encoded by the smoothened (SMO) gene acts as a positive mediator for Hedgehog signaling. This pathway regulates many cellular activities, developmental morphogenesis, and tumorigenesis. Using CRISPR/Cas9 to edit human embryonic stem cell line WA01 (H1), we established a SMO mutant cell line (WAe001-A-16). This cell line has a 40 bp homozygous deletion in exon 2 of SMO leading to a shift in the open reading frame and early termination at amino acid position 287. WAe001-A-16 maintains a normal karyotype, parental cell morphology, pluripotency markers, and the capacity to differentiate into all three germline layers.

Original languageEnglish
Pages (from-to)5-9
Number of pages5
JournalStem Cell Research
Volume27
DOIs
Publication statusPublished - Mar 2018
Externally publishedYes

Fingerprint

Dive into the research topics of 'Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing'. Together they form a unique fingerprint.

Cite this

Wu, F., Gao, G., Pan, T., Yang, Z., Xu, G., Abbas, N., Liu, Y., Chen, Y., Tan, S., You, K., Ke, X., Zhuang, Y., Lin, X., Yang, F., & Li, Y. X. (2018). Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing. Stem Cell Research, 27, 5-9. https://doi.org/10.1016/j.scr.2017.12.002