TY - JOUR
T1 - Feasibility Study on quantitative measurements of singlet oxygen generation using singlet oxygen sensor green
AU - Lin, Huiyun
AU - Shen, Yi
AU - Chen, Defu
AU - Lin, Lisheng
AU - Wilson, Brian C.
AU - Li, Buhong
AU - Xie, Shusen
PY - 2013/1
Y1 - 2013/1
N2 - The purpose of this study is to investigate the feasibility for quantitative measurement of singlet oxygen (1O2) generation by using a newly developed 1O2-specific fluorescence probe Singlet Oxygen Sensor Green reagent (SOSG). 1O2 generation from photoirradiation of a model photosensitizer Rose Bengal (RB), in initially air-statured phosphate buffered saline (PBS) was indirectly monitored with SOSG. In the presence of 1O2, SOSG can react with 1O2 to produce SOSG endoperoxides (SOSG-EP) that emit strong green fluorescence with the maximum at 531 nm. The green fluorescence of SOSG-EP is mainly dependent on the initial concentrations of RB and SOSG, and the photoirradiation time for 1O2 generation. Furthermore, kinetic analysis of the RB-sensitized photooxidation of SOSG is performed that, for the first time, allows quantitative measurement of 1O2 generation directly from the determination of reaction rate. In addition, the obtained 1O2 quantum yield of porphyrin-based photosensitizer hematoporphyrin monomethyl ether (HMME) in PBS by using SOSG is in good agreement with the value that independently determined by using direct measurement of 1O2 luminescence. The results of this study clearly demonstrate that the quantitative measurement of 1O 2 generation using SOSG can be achieved by determining the reaction rate with an appropriate measurement protocol.
AB - The purpose of this study is to investigate the feasibility for quantitative measurement of singlet oxygen (1O2) generation by using a newly developed 1O2-specific fluorescence probe Singlet Oxygen Sensor Green reagent (SOSG). 1O2 generation from photoirradiation of a model photosensitizer Rose Bengal (RB), in initially air-statured phosphate buffered saline (PBS) was indirectly monitored with SOSG. In the presence of 1O2, SOSG can react with 1O2 to produce SOSG endoperoxides (SOSG-EP) that emit strong green fluorescence with the maximum at 531 nm. The green fluorescence of SOSG-EP is mainly dependent on the initial concentrations of RB and SOSG, and the photoirradiation time for 1O2 generation. Furthermore, kinetic analysis of the RB-sensitized photooxidation of SOSG is performed that, for the first time, allows quantitative measurement of 1O2 generation directly from the determination of reaction rate. In addition, the obtained 1O2 quantum yield of porphyrin-based photosensitizer hematoporphyrin monomethyl ether (HMME) in PBS by using SOSG is in good agreement with the value that independently determined by using direct measurement of 1O2 luminescence. The results of this study clearly demonstrate that the quantitative measurement of 1O 2 generation using SOSG can be achieved by determining the reaction rate with an appropriate measurement protocol.
KW - Fluorescence probe
KW - Luminescence
KW - Quantitative measurement
KW - Quantum yield
KW - Singlet Oxygen Sensor Green reagent (SOSG)
KW - Singlet oxygen
UR - http://www.scopus.com/inward/record.url?scp=84873127531&partnerID=8YFLogxK
U2 - 10.1007/s10895-012-1114-5
DO - 10.1007/s10895-012-1114-5
M3 - Article
C2 - 22914972
AN - SCOPUS:84873127531
SN - 1053-0509
VL - 23
SP - 41
EP - 47
JO - Journal of Fluorescence
JF - Journal of Fluorescence
IS - 1
ER -