Extraction of antitumor component from fruiting body of ganoderma lucidum

Dongxu Zhao; Libo Wang; Xinlin Yang; Hesun Zhu

Extraction of antitumor component from fruiting body of ganoderma lucidum

Dongxu Zhao^*, Libo Wang, Xinlin Yang, Hesun Zhu

^*Corresponding author for this work

School of Life Science

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Aim To investigate the factors affecting the extraction of antitumor component from Ganoderma lucidum. Methods After being soaked in ethanol, the antitumor component was extracted by the method of percolation. The orthogonal design with table L₉(3⁴) was taken. The relative content of antitumor component in the extract was estimated by its harmfulness to HeLa cell. Results The extract inhibits the growth of HeLa cell in vitro. Among the 4 factors affecting the percolation, the operating temperature was the most significant one associated with the harmfulness and the solvent volume was the least. Conclusion There were some components of inhibiting the growth of Hela cell in the fruiting body of Ganoderma lucidum. The percolating conditions: the soaking time of 1 d, the percolating rate of 0. 5 mL · min^-1, the solvent volume of 200 mL and the temperature at 60 C, can ensure obtaining the best result of extraction.

Original language	English
Pages (from-to)	782-786
Number of pages	5
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	19
Issue number	6
Publication status	Published - 1999

Keywords

Antitumor component
Ganoderma lucidum
Orthogonal test
Percolation method

Cite this

Zhao, D., Wang, L., Yang, X., & Zhu, H. (1999). Extraction of antitumor component from fruiting body of ganoderma lucidum. Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology, 19(6), 782-786.

@article{56ed1e412cee4547b48ad12db86d7f1c,

title = "Extraction of antitumor component from fruiting body of ganoderma lucidum",

abstract = "Aim To investigate the factors affecting the extraction of antitumor component from Ganoderma lucidum. Methods After being soaked in ethanol, the antitumor component was extracted by the method of percolation. The orthogonal design with table L9(34) was taken. The relative content of antitumor component in the extract was estimated by its harmfulness to HeLa cell. Results The extract inhibits the growth of HeLa cell in vitro. Among the 4 factors affecting the percolation, the operating temperature was the most significant one associated with the harmfulness and the solvent volume was the least. Conclusion There were some components of inhibiting the growth of Hela cell in the fruiting body of Ganoderma lucidum. The percolating conditions: the soaking time of 1 d, the percolating rate of 0. 5 mL · min-1, the solvent volume of 200 mL and the temperature at 60 C, can ensure obtaining the best result of extraction.",

keywords = "Antitumor component, Ganoderma lucidum, Orthogonal test, Percolation method",

author = "Dongxu Zhao and Libo Wang and Xinlin Yang and Hesun Zhu",

year = "1999",

language = "English",

volume = "19",

pages = "782--786",

journal = "Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology",

issn = "1001-0645",

publisher = "Beijing Institute of Technology",

number = "6",

}

TY - JOUR

T1 - Extraction of antitumor component from fruiting body of ganoderma lucidum

AU - Zhao, Dongxu

AU - Wang, Libo

AU - Yang, Xinlin

AU - Zhu, Hesun

PY - 1999

Y1 - 1999

N2 - Aim To investigate the factors affecting the extraction of antitumor component from Ganoderma lucidum. Methods After being soaked in ethanol, the antitumor component was extracted by the method of percolation. The orthogonal design with table L9(34) was taken. The relative content of antitumor component in the extract was estimated by its harmfulness to HeLa cell. Results The extract inhibits the growth of HeLa cell in vitro. Among the 4 factors affecting the percolation, the operating temperature was the most significant one associated with the harmfulness and the solvent volume was the least. Conclusion There were some components of inhibiting the growth of Hela cell in the fruiting body of Ganoderma lucidum. The percolating conditions: the soaking time of 1 d, the percolating rate of 0. 5 mL · min-1, the solvent volume of 200 mL and the temperature at 60 C, can ensure obtaining the best result of extraction.

AB - Aim To investigate the factors affecting the extraction of antitumor component from Ganoderma lucidum. Methods After being soaked in ethanol, the antitumor component was extracted by the method of percolation. The orthogonal design with table L9(34) was taken. The relative content of antitumor component in the extract was estimated by its harmfulness to HeLa cell. Results The extract inhibits the growth of HeLa cell in vitro. Among the 4 factors affecting the percolation, the operating temperature was the most significant one associated with the harmfulness and the solvent volume was the least. Conclusion There were some components of inhibiting the growth of Hela cell in the fruiting body of Ganoderma lucidum. The percolating conditions: the soaking time of 1 d, the percolating rate of 0. 5 mL · min-1, the solvent volume of 200 mL and the temperature at 60 C, can ensure obtaining the best result of extraction.

KW - Antitumor component

KW - Ganoderma lucidum

KW - Orthogonal test

KW - Percolation method

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M3 - Article

AN - SCOPUS:71149103180

SN - 1001-0645

VL - 19

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JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

IS - 6

ER -

Extraction of antitumor component from fruiting body of ganoderma lucidum

Abstract

Keywords

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