Excitation wavelength analysis of a laser-induced fluorescence technique for quantification of extra virgin olive oil adulteration

The performance of the laser-induced fluorescence (LIF) technique is greatly affected by the excitation wavelength (EW). This study aims to find an appropriate EW that can be used for analyzing extra virgin olive oil (EVOO) adulteration quantification by comparing the effect of different EWs. The EWs of 405 nm, 450 nm, and 532 nm were selected to perform the comparative experiments. By using the three EWs in the experiments, the LIF spectra of EVOO samples adulterated with peanut oil (PO) or soybean oil (SO) in different proportions, as well as the prediction models established through different multivariate analysis algorithms were analyzed. The linear discriminant analysis (LDA) was applied for qualitative analysis, while the partial least squares regression (PLSR), backpropagation neural network, and k-nearest neighbor were employed for quantitative analysis. The results show that the performance of 450 nm EW is always superior to that of 405 and 532 nm EWs in any model, with a smaller root mean square error (RMSE). Using the LDA-PLSR model, the RMSE is 1.35% for SO adulterants and 1.36% for PO adulterants, respectively.