Evaluation of a rat model of Parkinson's disease by injection of 6-OHDA into the substantia nigra

Jian Mao; Wei Yang; Ran Wang; Yu Jing Luan; Hong Qing; Yu Lin Deng

doi:10.1109/ICCME.2007.4381977

Evaluation of a rat model of Parkinson's disease by injection of 6-OHDA into the substantia nigra

Jian Mao, Wei Yang, Ran Wang, Yu Jing Luan, Hong Qing, Yu Lin Deng^*

^*Corresponding author for this work

School of Medical and Technology

Beijing Institute of Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Citation (Scopus)

Abstract

A rat model of Parkinson's disease was established by direct injection of 6-OHDA into the substantia nigra unilaterally. Animals were assessed by D-amphetamine rotation test, anti-TH staining and detection of dopamine and its metabolites by HPLC-ECD. Rotational behavior experiment shown that the achievement ratio of PD model is 48.9%. Anti-TH staining result indicated that 6-OHDA treatment caused extensive loss of TH immunoreactivity in the substantia nigra and their projections to the striatum. Moreover, DA and its metabolites reduced remarkably in the substantia nigra and striatum of 6-OHDA lesioned rat model compared to control. Therefore, by evaluation of experimental animals, unilateral 6-OHDA induced substantia nigra degeneration can establish dependable rat model of Parkinson's disease with higher achievement ratio.

Original language	English
Title of host publication	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Pages	1413-1416
Number of pages	4
DOIs	https://doi.org/10.1109/ICCME.2007.4381977
Publication status	Published - 2007
Event	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 - Beijing, China Duration: 23 May 2007 → 27 May 2007

Publication series

Name	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

Conference

Conference	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Country/Territory	China
City	Beijing
Period	23/05/07 → 27/05/07

Access to Document

10.1109/ICCME.2007.4381977

Cite this

Mao, J., Yang, W., Wang, R., Luan, Y. J., Qing, H., & Deng, Y. L. (2007). Evaluation of a rat model of Parkinson's disease by injection of 6-OHDA into the substantia nigra. In 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 (pp. 1413-1416). Article 4381977 (2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007). https://doi.org/10.1109/ICCME.2007.4381977

@inproceedings{9c4f2b2a64154314accba287dd18f19a,

title = "Evaluation of a rat model of Parkinson's disease by injection of 6-OHDA into the substantia nigra",

abstract = "A rat model of Parkinson's disease was established by direct injection of 6-OHDA into the substantia nigra unilaterally. Animals were assessed by D-amphetamine rotation test, anti-TH staining and detection of dopamine and its metabolites by HPLC-ECD. Rotational behavior experiment shown that the achievement ratio of PD model is 48.9%. Anti-TH staining result indicated that 6-OHDA treatment caused extensive loss of TH immunoreactivity in the substantia nigra and their projections to the striatum. Moreover, DA and its metabolites reduced remarkably in the substantia nigra and striatum of 6-OHDA lesioned rat model compared to control. Therefore, by evaluation of experimental animals, unilateral 6-OHDA induced substantia nigra degeneration can establish dependable rat model of Parkinson's disease with higher achievement ratio.",

author = "Jian Mao and Wei Yang and Ran Wang and Luan, {Yu Jing} and Hong Qing and Deng, {Yu Lin}",

year = "2007",

doi = "10.1109/ICCME.2007.4381977",

language = "English",

isbn = "1424410789",

series = "2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007",

pages = "1413--1416",

booktitle = "2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007",

note = "2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 ; Conference date: 23-05-2007 Through 27-05-2007",

}

Mao, J, Yang, W, Wang, R, Luan, YJ, Qing, H & Deng, YL 2007, Evaluation of a rat model of Parkinson's disease by injection of 6-OHDA into the substantia nigra. in 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007., 4381977, 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007, pp. 1413-1416, 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007, Beijing, China, 23/05/07. https://doi.org/10.1109/ICCME.2007.4381977

Evaluation of a rat model of Parkinson's disease by injection of 6-OHDA into the substantia nigra. / Mao, Jian; Yang, Wei; Wang, Ran et al.
2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007. 2007. p. 1413-1416 4381977 (2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Evaluation of a rat model of Parkinson's disease by injection of 6-OHDA into the substantia nigra

AU - Mao, Jian

AU - Yang, Wei

AU - Wang, Ran

AU - Luan, Yu Jing

AU - Qing, Hong

AU - Deng, Yu Lin

PY - 2007

Y1 - 2007

N2 - A rat model of Parkinson's disease was established by direct injection of 6-OHDA into the substantia nigra unilaterally. Animals were assessed by D-amphetamine rotation test, anti-TH staining and detection of dopamine and its metabolites by HPLC-ECD. Rotational behavior experiment shown that the achievement ratio of PD model is 48.9%. Anti-TH staining result indicated that 6-OHDA treatment caused extensive loss of TH immunoreactivity in the substantia nigra and their projections to the striatum. Moreover, DA and its metabolites reduced remarkably in the substantia nigra and striatum of 6-OHDA lesioned rat model compared to control. Therefore, by evaluation of experimental animals, unilateral 6-OHDA induced substantia nigra degeneration can establish dependable rat model of Parkinson's disease with higher achievement ratio.

AB - A rat model of Parkinson's disease was established by direct injection of 6-OHDA into the substantia nigra unilaterally. Animals were assessed by D-amphetamine rotation test, anti-TH staining and detection of dopamine and its metabolites by HPLC-ECD. Rotational behavior experiment shown that the achievement ratio of PD model is 48.9%. Anti-TH staining result indicated that 6-OHDA treatment caused extensive loss of TH immunoreactivity in the substantia nigra and their projections to the striatum. Moreover, DA and its metabolites reduced remarkably in the substantia nigra and striatum of 6-OHDA lesioned rat model compared to control. Therefore, by evaluation of experimental animals, unilateral 6-OHDA induced substantia nigra degeneration can establish dependable rat model of Parkinson's disease with higher achievement ratio.

UR - http://www.scopus.com/inward/record.url?scp=48149110287&partnerID=8YFLogxK

U2 - 10.1109/ICCME.2007.4381977

DO - 10.1109/ICCME.2007.4381977

M3 - Conference contribution

AN - SCOPUS:48149110287

SN - 1424410789

SN - 9781424410781

T3 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

SP - 1413

EP - 1416

BT - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

T2 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

Y2 - 23 May 2007 through 27 May 2007

ER -

Mao J, Yang W, Wang R, Luan YJ, Qing H, Deng YL. Evaluation of a rat model of Parkinson's disease by injection of 6-OHDA into the substantia nigra. In 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007. 2007. p. 1413-1416. 4381977. (2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007). doi: 10.1109/ICCME.2007.4381977

Evaluation of a rat model of Parkinson's disease by injection of 6-OHDA into the substantia nigra

Abstract

Publication series

Conference

Access to Document

Other files and links

Fingerprint

Cite this