Enhanced transfection of human mesenchymal stem cells using a hyaluronic acid/calcium phosphate hybrid gene delivery system

Jung Eun Lee; Yue Yin; Su Yeon Lim; E. Seul Kim; Jaeback Jung; Dahwun Kim; Ji Won Park; Min Sang Lee; Ji Hoon Jeong

doi:10.3390/polym11050798

Enhanced transfection of human mesenchymal stem cells using a hyaluronic acid/calcium phosphate hybrid gene delivery system

Jung Eun Lee, Yue Yin, Su Yeon Lim, E. Seul Kim, Jaeback Jung, Dahwun Kim, Ji Won Park, Min Sang Lee^*, Ji Hoon Jeong

^*Corresponding author for this work

Sungkyunkwan University

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

Human mesenchymal stem cells (hMSCs) show enormous potential in regenerative medicine and tissue engineering. However, current use of hMSCs in clinics is still limited because there is no appropriate way to control their behavior in vivo, such as differentiation to a desired cell type. Genetic modification may provide an opportunity to control the cells in an active manner. One of the major hurdles for genetic manipulation of hMSCs is the lack of an efficient and safe gene delivery system. Herein, biocompatible calcium phosphate (CaP)-based nanoparticles stabilized with a catechol-derivatized hyaluronic acid (dopa-HA) conjugate were used as a carrier for gene transfection to hMSCs for improved differentiation. Owing to the specific interactions between HA and CD44 of bone marrow-derived hMSCs, dopa-HA/CaP showed significantly higher transfection in hMSCs than branched polyethylenimine (bPEI, MW 25 kDa) with no cytotoxicity. The co-delivery of a plasmid DNA encoding bone morphogenetic protein 2 (BMP-2 pDNA) and micro RNA 148b (miRNA-148b) by dopa-HA/CaP achieved significantly improved osteogenic differentiation of hMSCs.

Original language	English
Article number	798
Journal	Polymers
Volume	11
Issue number	5
DOIs	https://doi.org/10.3390/polym11050798
Publication status	Published - 1 May 2019
Externally published	Yes

Keywords

Calcium phosphate nanoparticle
Catechol modified
Gene delivery
Human mesenchymal stem cell
Hyaluronic acid
Osteogenic differentiation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Enhanced transfection of human mesenchymal stem cells using a hyaluronic acid/calcium phosphate hybrid gene delivery system",

abstract = "Human mesenchymal stem cells (hMSCs) show enormous potential in regenerative medicine and tissue engineering. However, current use of hMSCs in clinics is still limited because there is no appropriate way to control their behavior in vivo, such as differentiation to a desired cell type. Genetic modification may provide an opportunity to control the cells in an active manner. One of the major hurdles for genetic manipulation of hMSCs is the lack of an efficient and safe gene delivery system. Herein, biocompatible calcium phosphate (CaP)-based nanoparticles stabilized with a catechol-derivatized hyaluronic acid (dopa-HA) conjugate were used as a carrier for gene transfection to hMSCs for improved differentiation. Owing to the specific interactions between HA and CD44 of bone marrow-derived hMSCs, dopa-HA/CaP showed significantly higher transfection in hMSCs than branched polyethylenimine (bPEI, MW 25 kDa) with no cytotoxicity. The co-delivery of a plasmid DNA encoding bone morphogenetic protein 2 (BMP-2 pDNA) and micro RNA 148b (miRNA-148b) by dopa-HA/CaP achieved significantly improved osteogenic differentiation of hMSCs.",

keywords = "Calcium phosphate nanoparticle, Catechol modified, Gene delivery, Human mesenchymal stem cell, Hyaluronic acid, Osteogenic differentiation",

author = "Lee, {Jung Eun} and Yue Yin and Lim, {Su Yeon} and Kim, {E. Seul} and Jaeback Jung and Dahwun Kim and Park, {Ji Won} and Lee, {Min Sang} and Jeong, {Ji Hoon}",

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T1 - Enhanced transfection of human mesenchymal stem cells using a hyaluronic acid/calcium phosphate hybrid gene delivery system

AU - Lee, Jung Eun

AU - Yin, Yue

AU - Lim, Su Yeon

AU - Kim, E. Seul

AU - Jung, Jaeback

AU - Kim, Dahwun

AU - Park, Ji Won

AU - Lee, Min Sang

AU - Jeong, Ji Hoon

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Human mesenchymal stem cells (hMSCs) show enormous potential in regenerative medicine and tissue engineering. However, current use of hMSCs in clinics is still limited because there is no appropriate way to control their behavior in vivo, such as differentiation to a desired cell type. Genetic modification may provide an opportunity to control the cells in an active manner. One of the major hurdles for genetic manipulation of hMSCs is the lack of an efficient and safe gene delivery system. Herein, biocompatible calcium phosphate (CaP)-based nanoparticles stabilized with a catechol-derivatized hyaluronic acid (dopa-HA) conjugate were used as a carrier for gene transfection to hMSCs for improved differentiation. Owing to the specific interactions between HA and CD44 of bone marrow-derived hMSCs, dopa-HA/CaP showed significantly higher transfection in hMSCs than branched polyethylenimine (bPEI, MW 25 kDa) with no cytotoxicity. The co-delivery of a plasmid DNA encoding bone morphogenetic protein 2 (BMP-2 pDNA) and micro RNA 148b (miRNA-148b) by dopa-HA/CaP achieved significantly improved osteogenic differentiation of hMSCs.

AB - Human mesenchymal stem cells (hMSCs) show enormous potential in regenerative medicine and tissue engineering. However, current use of hMSCs in clinics is still limited because there is no appropriate way to control their behavior in vivo, such as differentiation to a desired cell type. Genetic modification may provide an opportunity to control the cells in an active manner. One of the major hurdles for genetic manipulation of hMSCs is the lack of an efficient and safe gene delivery system. Herein, biocompatible calcium phosphate (CaP)-based nanoparticles stabilized with a catechol-derivatized hyaluronic acid (dopa-HA) conjugate were used as a carrier for gene transfection to hMSCs for improved differentiation. Owing to the specific interactions between HA and CD44 of bone marrow-derived hMSCs, dopa-HA/CaP showed significantly higher transfection in hMSCs than branched polyethylenimine (bPEI, MW 25 kDa) with no cytotoxicity. The co-delivery of a plasmid DNA encoding bone morphogenetic protein 2 (BMP-2 pDNA) and micro RNA 148b (miRNA-148b) by dopa-HA/CaP achieved significantly improved osteogenic differentiation of hMSCs.

KW - Calcium phosphate nanoparticle

KW - Catechol modified

KW - Gene delivery

KW - Human mesenchymal stem cell

KW - Hyaluronic acid

KW - Osteogenic differentiation

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Enhanced transfection of human mesenchymal stem cells using a hyaluronic acid/calcium phosphate hybrid gene delivery system

Abstract

Keywords

UN SDGs

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