Endoscope-based beveled and volume fiber-optic Raman probes for in vivo diagnosis of gastric dysplasia: A comparative study

Jianfeng Wang, Kan Lin, Wei Zheng, Khek Yu Ho, Ming Teh, Khay Guan Yeoh, Zhiwei Huang*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Raman spectroscopy is a unique optical vibrational spectroscopic technique which is capable of probing biochemical and biomolecular structures and conformations associated with disease transformation. Raman probe is a key component to facilitate the in vivo tissue diagnosis by using Raman spectroscopy. The diagnostic performance of the two different endoscope-based fiber-optic Raman probe designs (i.e., beveled and volume Raman probes) were evaluated for real-time, in vivo diagnosis of gastric dysplasia at endoscopy. The beveled Raman probe provides approximately 2-fold improvements in tissue Raman to autofluorescence intensity ratios as compared to the use of volume Raman probe. The diagnostic accuracy of gastric dysplasia using beveled Raman probe is 93.0% (sensitivity of 92.5%; specificity of 93.1%), which is superior to the diagnostic performance (accuracy of 88.4%; sensitivity of 85.8%; specificity of 88.6%) using the volume Raman probe. Biomolecular modeling is finally employed to extract the different Raman active components interrogated by the two types of endoscope-based Raman probes.

Original languageEnglish
Title of host publicationBiomedical Vibrational Spectroscopy 2016
Subtitle of host publicationAdvances in Research and Industry
EditorsAnita Mahadevan-Jansen, Wolfgang Petrich
PublisherSPIE
ISBN (Electronic)9781628419382
DOIs
Publication statusPublished - 2016
Externally publishedYes
EventBiomedical Vibrational Spectroscopy 2016: Advances in Research and Industry - San Francisco, United States
Duration: 13 Feb 201614 Feb 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9704
ISSN (Print)1605-7422

Conference

ConferenceBiomedical Vibrational Spectroscopy 2016: Advances in Research and Industry
Country/TerritoryUnited States
CitySan Francisco
Period13/02/1614/02/16

Keywords

  • Endoscopy
  • Fiber-optic Raman probe
  • Gastric dysplasia
  • In vivo optical diagnosis
  • Raman spectroscopy

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