Elimination pathways of systemically delivered siRNA

Yuanyu Huang; Junmei Hong; Shuquan Zheng; Yi Ding; Shutao Guo; Hongyan Zhang; Xiuqin Zhang; Quan Du; Zicai Liang

doi:10.1038/mt.2010.266

Elimination pathways of systemically delivered siRNA

Yuanyu Huang, Junmei Hong, Shuquan Zheng, Yi Ding, Shutao Guo, Hongyan Zhang, Xiuqin Zhang, Quan Du^*, Zicai Liang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

129 Citations (Scopus)

Abstract

The elimination process of systemically administered small interfering RNA (siRNA) was investigated by using siRNA labeled with an infrared fluorescent dye. A novel siRNA elimination pathway was identified. In this pathway, liver-enriched siRNA is secreted into the gallbladder and then emptied into the intestine. Blocking this pathway resulted in the absence of siRNA fluorescence within the intestine, with greatly enhanced siRNA accumulation in liver and gallbladder at the same time. Furthermore, we demonstrated that delivery carriers play an essential role in siRNA distribution and elimination, highlighting their importance in siRNA therapeutics.

Original language	English
Pages (from-to)	381-385
Number of pages	5
Journal	Molecular Therapy
Volume	19
Issue number	2
DOIs	https://doi.org/10.1038/mt.2010.266
Publication status	Published - Feb 2011
Externally published	Yes

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10.1038/mt.2010.266

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abstract = "The elimination process of systemically administered small interfering RNA (siRNA) was investigated by using siRNA labeled with an infrared fluorescent dye. A novel siRNA elimination pathway was identified. In this pathway, liver-enriched siRNA is secreted into the gallbladder and then emptied into the intestine. Blocking this pathway resulted in the absence of siRNA fluorescence within the intestine, with greatly enhanced siRNA accumulation in liver and gallbladder at the same time. Furthermore, we demonstrated that delivery carriers play an essential role in siRNA distribution and elimination, highlighting their importance in siRNA therapeutics.",

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AU - Hong, Junmei

AU - Zheng, Shuquan

AU - Ding, Yi

AU - Guo, Shutao

AU - Zhang, Hongyan

AU - Zhang, Xiuqin

AU - Du, Quan

AU - Liang, Zicai

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AB - The elimination process of systemically administered small interfering RNA (siRNA) was investigated by using siRNA labeled with an infrared fluorescent dye. A novel siRNA elimination pathway was identified. In this pathway, liver-enriched siRNA is secreted into the gallbladder and then emptied into the intestine. Blocking this pathway resulted in the absence of siRNA fluorescence within the intestine, with greatly enhanced siRNA accumulation in liver and gallbladder at the same time. Furthermore, we demonstrated that delivery carriers play an essential role in siRNA distribution and elimination, highlighting their importance in siRNA therapeutics.

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Elimination pathways of systemically delivered siRNA

Abstract

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