TY - GEN
T1 - Efficient and high-throughput electroporation chips
AU - Li, Zhihong
AU - Wei, Zewen
AU - Li, Xueming
AU - Du, Quan
AU - Liang, Zicai
PY - 2011
Y1 - 2011
N2 - To fulfill demands of high efficient in vitro and in vivo nucleic acid delivery, three types of electroporation microchips were developed. To better fit the standard multi-well plate, we designed a chip with annual interdigital electrodes which generate even electric field. High efficient DNA and siRNA delivery was achieved on both supernatant and adherent cells. The parylene based substrate was employed to realize in vivo electroporation. The flexible film fits the natural shape of the electroporated objects and has good biocompatibility, which makes the chip potential for implantable electroporation devices. Besides, to meet requirements of continuous cell treatment, we designed a flow-type electroporation chip, in which the sheath flow was employed to isolate cells from harmful effects due to water electrolysis around electrodes.
AB - To fulfill demands of high efficient in vitro and in vivo nucleic acid delivery, three types of electroporation microchips were developed. To better fit the standard multi-well plate, we designed a chip with annual interdigital electrodes which generate even electric field. High efficient DNA and siRNA delivery was achieved on both supernatant and adherent cells. The parylene based substrate was employed to realize in vivo electroporation. The flexible film fits the natural shape of the electroporated objects and has good biocompatibility, which makes the chip potential for implantable electroporation devices. Besides, to meet requirements of continuous cell treatment, we designed a flow-type electroporation chip, in which the sheath flow was employed to isolate cells from harmful effects due to water electrolysis around electrodes.
KW - Electroporation
KW - in vivo gene delivery
KW - microfluidics
KW - siRNA delivery
UR - http://www.scopus.com/inward/record.url?scp=80052126439&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2011.5969892
DO - 10.1109/TRANSDUCERS.2011.5969892
M3 - Conference contribution
AN - SCOPUS:80052126439
SN - 9781457701573
T3 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
SP - 1825
EP - 1828
BT - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
T2 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Y2 - 5 June 2011 through 9 June 2011
ER -
