Dual Functional Ultrasensitive Point-of-Care Clinical Diagnosis Using Metal-Organic Frameworks-Based Immunobeads

Fengli Chai; Dou Wang; Fei Shi; Wenfu Zheng; Xiaohui Zhao; Yao Chen; Cuiping Mao; Jiangjiang Zhang; Xingyu Jiang

doi:10.1021/acs.nanolett.3c02828

Dual Functional Ultrasensitive Point-of-Care Clinical Diagnosis Using Metal-Organic Frameworks-Based Immunobeads

Fengli Chai, Dou Wang, Fei Shi, Wenfu Zheng, Xiaohui Zhao, Yao Chen, Cuiping Mao, Jiangjiang Zhang^*, Xingyu Jiang^*

^*Corresponding author for this work

School of Life Science

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Sepsis is an acute systemic infectious syndrome with high fatality. Fast and accurate diagnosis, monitoring, and medication of sepsis are essential. We exploited the fluorescent metal-AIEgen frameworks (MAFs) and demonstrated the dual functions of protein detection and bacteria identification: (i) ultrasensitive point-of-care (POC) detection of sepsis biomarkers (100 times enhanced sensitivity); (ii) rapid POC identification of Gram-negative/positive bacteria (selective aggregation within 20 min). Fluorescent lateral flow immunoassays (LFAs) are convenient and inexpensive for POC tests. MAFs possess a large surface area, excellent photostability, high quantum yield (∼80%), and multiple active sites serving as protein binding domains for ultrasensitive detection of sepsis biomarkers (IL-6/PCT) on LFAs. The limit of detection (LOD) for IL-6/PCT is 0.252/0.333 pg/mL. Rapid appraisal of infectious bacteria is vital to guide the use of medicines. The dual-functional fluorescent MAFs have great potential in POC tests for the clinical diagnosis of bacterial infections.

Original language	English
Pages (from-to)	9056-9064
Number of pages	9
Journal	Nano Letters
Volume	23
Issue number	19
DOIs	https://doi.org/10.1021/acs.nanolett.3c02828
Publication status	Published - 11 Oct 2023

Keywords

Bacterial recognition
Inflammation factor detections
Lateral flow immunoassays
Metal−organic frameworks
Sepsis diagnosis

Access to Document

10.1021/acs.nanolett.3c02828

Cite this

Chai, F., Wang, D., Shi, F., Zheng, W., Zhao, X., Chen, Y., Mao, C., Zhang, J., & Jiang, X. (2023). Dual Functional Ultrasensitive Point-of-Care Clinical Diagnosis Using Metal-Organic Frameworks-Based Immunobeads. Nano Letters, 23(19), 9056-9064. https://doi.org/10.1021/acs.nanolett.3c02828

@article{cd9cbb42c27a418ba7f0f777bfb5d071,

title = "Dual Functional Ultrasensitive Point-of-Care Clinical Diagnosis Using Metal-Organic Frameworks-Based Immunobeads",

abstract = "Sepsis is an acute systemic infectious syndrome with high fatality. Fast and accurate diagnosis, monitoring, and medication of sepsis are essential. We exploited the fluorescent metal-AIEgen frameworks (MAFs) and demonstrated the dual functions of protein detection and bacteria identification: (i) ultrasensitive point-of-care (POC) detection of sepsis biomarkers (100 times enhanced sensitivity); (ii) rapid POC identification of Gram-negative/positive bacteria (selective aggregation within 20 min). Fluorescent lateral flow immunoassays (LFAs) are convenient and inexpensive for POC tests. MAFs possess a large surface area, excellent photostability, high quantum yield (∼80%), and multiple active sites serving as protein binding domains for ultrasensitive detection of sepsis biomarkers (IL-6/PCT) on LFAs. The limit of detection (LOD) for IL-6/PCT is 0.252/0.333 pg/mL. Rapid appraisal of infectious bacteria is vital to guide the use of medicines. The dual-functional fluorescent MAFs have great potential in POC tests for the clinical diagnosis of bacterial infections.",

keywords = "Bacterial recognition, Inflammation factor detections, Lateral flow immunoassays, Metal−organic frameworks, Sepsis diagnosis",

author = "Fengli Chai and Dou Wang and Fei Shi and Wenfu Zheng and Xiaohui Zhao and Yao Chen and Cuiping Mao and Jiangjiang Zhang and Xingyu Jiang",

note = "Publisher Copyright: {\textcopyright} 2023 American Chemical Society.",

year = "2023",

month = oct,

day = "11",

doi = "10.1021/acs.nanolett.3c02828",

language = "English",

volume = "23",

pages = "9056--9064",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

number = "19",

}

TY - JOUR

T1 - Dual Functional Ultrasensitive Point-of-Care Clinical Diagnosis Using Metal-Organic Frameworks-Based Immunobeads

AU - Chai, Fengli

AU - Wang, Dou

AU - Shi, Fei

AU - Zheng, Wenfu

AU - Zhao, Xiaohui

AU - Chen, Yao

AU - Mao, Cuiping

AU - Zhang, Jiangjiang

AU - Jiang, Xingyu

PY - 2023/10/11

Y1 - 2023/10/11

N2 - Sepsis is an acute systemic infectious syndrome with high fatality. Fast and accurate diagnosis, monitoring, and medication of sepsis are essential. We exploited the fluorescent metal-AIEgen frameworks (MAFs) and demonstrated the dual functions of protein detection and bacteria identification: (i) ultrasensitive point-of-care (POC) detection of sepsis biomarkers (100 times enhanced sensitivity); (ii) rapid POC identification of Gram-negative/positive bacteria (selective aggregation within 20 min). Fluorescent lateral flow immunoassays (LFAs) are convenient and inexpensive for POC tests. MAFs possess a large surface area, excellent photostability, high quantum yield (∼80%), and multiple active sites serving as protein binding domains for ultrasensitive detection of sepsis biomarkers (IL-6/PCT) on LFAs. The limit of detection (LOD) for IL-6/PCT is 0.252/0.333 pg/mL. Rapid appraisal of infectious bacteria is vital to guide the use of medicines. The dual-functional fluorescent MAFs have great potential in POC tests for the clinical diagnosis of bacterial infections.

AB - Sepsis is an acute systemic infectious syndrome with high fatality. Fast and accurate diagnosis, monitoring, and medication of sepsis are essential. We exploited the fluorescent metal-AIEgen frameworks (MAFs) and demonstrated the dual functions of protein detection and bacteria identification: (i) ultrasensitive point-of-care (POC) detection of sepsis biomarkers (100 times enhanced sensitivity); (ii) rapid POC identification of Gram-negative/positive bacteria (selective aggregation within 20 min). Fluorescent lateral flow immunoassays (LFAs) are convenient and inexpensive for POC tests. MAFs possess a large surface area, excellent photostability, high quantum yield (∼80%), and multiple active sites serving as protein binding domains for ultrasensitive detection of sepsis biomarkers (IL-6/PCT) on LFAs. The limit of detection (LOD) for IL-6/PCT is 0.252/0.333 pg/mL. Rapid appraisal of infectious bacteria is vital to guide the use of medicines. The dual-functional fluorescent MAFs have great potential in POC tests for the clinical diagnosis of bacterial infections.

KW - Bacterial recognition

KW - Inflammation factor detections

KW - Lateral flow immunoassays

KW - Metal−organic frameworks

KW - Sepsis diagnosis

UR - http://www.scopus.com/inward/record.url?scp=85175250990&partnerID=8YFLogxK

U2 - 10.1021/acs.nanolett.3c02828

DO - 10.1021/acs.nanolett.3c02828

M3 - Article

AN - SCOPUS:85175250990

SN - 1530-6984

VL - 23

SP - 9056

EP - 9064

JO - Nano Letters

JF - Nano Letters

IS - 19

ER -

Dual Functional Ultrasensitive Point-of-Care Clinical Diagnosis Using Metal-Organic Frameworks-Based Immunobeads

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this