Drosophila Trf4-1 involves in mRNA and primary miRNA transcription

Yongxiang Liu; Ming Wang; Xin Liu; Jia Quan; Yuan Fang; Yujie Liu; Yang Qiu; Yang Yu; Xi Zhou

doi:10.1016/j.bbrc.2019.02.129

Drosophila Trf4-1 involves in mRNA and primary miRNA transcription

Yongxiang Liu, Ming Wang, Xin Liu, Jia Quan, Yuan Fang, Yujie Liu, Yang Qiu, Yang Yu, Xi Zhou^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Drosophila Trf4-1 (DmTrf4-1) is a polyadenylation polymerase or terminal nucleotidyl transferase (PAP/TENT) that has been reported to add poly(A) tails to snRNAs in nucleus or mRNAs in cytoplasm. Here, we found that the loss of Trf4-1 resulted in the reduction of mRNAs and primary miRNAs (pri-miRNAs) in both Drosophila S2 cells and adult flies. Interestingly, the role of Trf4-1 in transcription is independent of its PAP/TENT activity. Moreover, using the chromatin immunoprecipitation assay, we uncovered that the loss of Trf4-1 led to abnormal RNA polymerase II accumulation and reduced H3K4me3 binding in promoter regions. Thus, our study indicates a positive role of Trf4-1 in the transcription of mRNAs and pri-miRNAs.

Original language	English
Pages (from-to)	806-812
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	511
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2019.02.129
Publication status	Published - 16 Apr 2019
Externally published	Yes

Keywords

H3K4me3
RNA Po II
Trf4-1
mRNA transcription
pri-miRNA transcription

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10.1016/j.bbrc.2019.02.129

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title = "Drosophila Trf4-1 involves in mRNA and primary miRNA transcription",

abstract = "Drosophila Trf4-1 (DmTrf4-1) is a polyadenylation polymerase or terminal nucleotidyl transferase (PAP/TENT) that has been reported to add poly(A) tails to snRNAs in nucleus or mRNAs in cytoplasm. Here, we found that the loss of Trf4-1 resulted in the reduction of mRNAs and primary miRNAs (pri-miRNAs) in both Drosophila S2 cells and adult flies. Interestingly, the role of Trf4-1 in transcription is independent of its PAP/TENT activity. Moreover, using the chromatin immunoprecipitation assay, we uncovered that the loss of Trf4-1 led to abnormal RNA polymerase II accumulation and reduced H3K4me3 binding in promoter regions. Thus, our study indicates a positive role of Trf4-1 in the transcription of mRNAs and pri-miRNAs.",

keywords = "H3K4me3, RNA Po II, Trf4-1, mRNA transcription, pri-miRNA transcription",

author = "Yongxiang Liu and Ming Wang and Xin Liu and Jia Quan and Yuan Fang and Yujie Liu and Yang Qiu and Yang Yu and Xi Zhou",

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AU - Liu, Yongxiang

AU - Wang, Ming

AU - Liu, Xin

AU - Quan, Jia

AU - Fang, Yuan

AU - Liu, Yujie

AU - Qiu, Yang

AU - Yu, Yang

AU - Zhou, Xi

PY - 2019/4/16

Y1 - 2019/4/16

N2 - Drosophila Trf4-1 (DmTrf4-1) is a polyadenylation polymerase or terminal nucleotidyl transferase (PAP/TENT) that has been reported to add poly(A) tails to snRNAs in nucleus or mRNAs in cytoplasm. Here, we found that the loss of Trf4-1 resulted in the reduction of mRNAs and primary miRNAs (pri-miRNAs) in both Drosophila S2 cells and adult flies. Interestingly, the role of Trf4-1 in transcription is independent of its PAP/TENT activity. Moreover, using the chromatin immunoprecipitation assay, we uncovered that the loss of Trf4-1 led to abnormal RNA polymerase II accumulation and reduced H3K4me3 binding in promoter regions. Thus, our study indicates a positive role of Trf4-1 in the transcription of mRNAs and pri-miRNAs.

AB - Drosophila Trf4-1 (DmTrf4-1) is a polyadenylation polymerase or terminal nucleotidyl transferase (PAP/TENT) that has been reported to add poly(A) tails to snRNAs in nucleus or mRNAs in cytoplasm. Here, we found that the loss of Trf4-1 resulted in the reduction of mRNAs and primary miRNAs (pri-miRNAs) in both Drosophila S2 cells and adult flies. Interestingly, the role of Trf4-1 in transcription is independent of its PAP/TENT activity. Moreover, using the chromatin immunoprecipitation assay, we uncovered that the loss of Trf4-1 led to abnormal RNA polymerase II accumulation and reduced H3K4me3 binding in promoter regions. Thus, our study indicates a positive role of Trf4-1 in the transcription of mRNAs and pri-miRNAs.

KW - H3K4me3

KW - RNA Po II

KW - Trf4-1

KW - mRNA transcription

KW - pri-miRNA transcription

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EP - 812

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Drosophila Trf4-1 involves in mRNA and primary miRNA transcription

Abstract

Keywords

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