Diminished representation of vitamin-B12-producing bacteria in constipated elders with frailty

Luqing Zhao; Xin Deng; Ning Ding; Yanzhen Liu; Yun Ma; Jiake Li; Shuqing Wang; Xingyu Ji; Jinjuan Wang; Dianpeng Wang; Yanhui Li; Dan Dou; Xihan Zhu; Lixin Zhu; Shengsheng Zhang

doi:10.1016/j.isci.2024.110403

Diminished representation of vitamin-B12-producing bacteria in constipated elders with frailty

Luqing Zhao, Xin Deng, Ning Ding, Yanzhen Liu, Yun Ma, Jiake Li, Shuqing Wang, Xingyu Ji, Jinjuan Wang, Dianpeng Wang, Yanhui Li, Dan Dou, Xihan Zhu, Lixin Zhu^*, Shengsheng Zhang^*

^*Corresponding author for this work

School of Mathematics and Statistics

Research output: Contribution to journal › Article › peer-review

Abstract

Constipation and frailty are associated with intestinal dysbiosis. This study aims to identify intestinal microbial signatures that can differentiate between constipated elders accompanied by frailty and those without frailty. We collected stool samples from 61 participants and conducted 16S rRNA gene sequencing. Constipated patients with frailty (Constipation_F) exhibited reduced gut microbial diversities compared to constipated patients without frailty (Constipation_NF) and healthy individuals (C). From differential genera, random forest models identified 14, 8, and 5 biomarkers for distinguishing Constipation_F from Constipation_NF, Constipation_F from C, and Constipation_NF from C, respectively. Functional analysis revealed that pathways (P381-PWY and PWY-5507) related to vitamin B12 synthesis were reduced in Constipation_F, which aligns with the decreased abundances of vitamin-B12-producing Actinomyces and Akkermansia in this group. Our study unveils substantial differences in gut microbiota between constipated elders with frailty and those without, underscoring the diagnostic and therapeutic potential of genera involved in vitamin B12 synthesis.

Original language	English
Article number	110403
Journal	iScience
Volume	27
Issue number	8
DOIs	https://doi.org/10.1016/j.isci.2024.110403
Publication status	Published - 16 Aug 2024

Keywords

Microbial metabolism
Microbiology

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10.1016/j.isci.2024.110403

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title = "Diminished representation of vitamin-B12-producing bacteria in constipated elders with frailty",

abstract = "Constipation and frailty are associated with intestinal dysbiosis. This study aims to identify intestinal microbial signatures that can differentiate between constipated elders accompanied by frailty and those without frailty. We collected stool samples from 61 participants and conducted 16S rRNA gene sequencing. Constipated patients with frailty (Constipation_F) exhibited reduced gut microbial diversities compared to constipated patients without frailty (Constipation_NF) and healthy individuals (C). From differential genera, random forest models identified 14, 8, and 5 biomarkers for distinguishing Constipation_F from Constipation_NF, Constipation_F from C, and Constipation_NF from C, respectively. Functional analysis revealed that pathways (P381-PWY and PWY-5507) related to vitamin B12 synthesis were reduced in Constipation_F, which aligns with the decreased abundances of vitamin-B12-producing Actinomyces and Akkermansia in this group. Our study unveils substantial differences in gut microbiota between constipated elders with frailty and those without, underscoring the diagnostic and therapeutic potential of genera involved in vitamin B12 synthesis.",

keywords = "Microbial metabolism, Microbiology",

author = "Luqing Zhao and Xin Deng and Ning Ding and Yanzhen Liu and Yun Ma and Jiake Li and Shuqing Wang and Xingyu Ji and Jinjuan Wang and Dianpeng Wang and Yanhui Li and Dan Dou and Xihan Zhu and Lixin Zhu and Shengsheng Zhang",

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year = "2024",

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doi = "10.1016/j.isci.2024.110403",

language = "English",

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T1 - Diminished representation of vitamin-B12-producing bacteria in constipated elders with frailty

AU - Zhao, Luqing

AU - Deng, Xin

AU - Ding, Ning

AU - Liu, Yanzhen

AU - Ma, Yun

AU - Li, Jiake

AU - Wang, Shuqing

AU - Ji, Xingyu

AU - Wang, Jinjuan

AU - Wang, Dianpeng

AU - Li, Yanhui

AU - Dou, Dan

AU - Zhu, Xihan

AU - Zhu, Lixin

AU - Zhang, Shengsheng

PY - 2024/8/16

Y1 - 2024/8/16

N2 - Constipation and frailty are associated with intestinal dysbiosis. This study aims to identify intestinal microbial signatures that can differentiate between constipated elders accompanied by frailty and those without frailty. We collected stool samples from 61 participants and conducted 16S rRNA gene sequencing. Constipated patients with frailty (Constipation_F) exhibited reduced gut microbial diversities compared to constipated patients without frailty (Constipation_NF) and healthy individuals (C). From differential genera, random forest models identified 14, 8, and 5 biomarkers for distinguishing Constipation_F from Constipation_NF, Constipation_F from C, and Constipation_NF from C, respectively. Functional analysis revealed that pathways (P381-PWY and PWY-5507) related to vitamin B12 synthesis were reduced in Constipation_F, which aligns with the decreased abundances of vitamin-B12-producing Actinomyces and Akkermansia in this group. Our study unveils substantial differences in gut microbiota between constipated elders with frailty and those without, underscoring the diagnostic and therapeutic potential of genera involved in vitamin B12 synthesis.

AB - Constipation and frailty are associated with intestinal dysbiosis. This study aims to identify intestinal microbial signatures that can differentiate between constipated elders accompanied by frailty and those without frailty. We collected stool samples from 61 participants and conducted 16S rRNA gene sequencing. Constipated patients with frailty (Constipation_F) exhibited reduced gut microbial diversities compared to constipated patients without frailty (Constipation_NF) and healthy individuals (C). From differential genera, random forest models identified 14, 8, and 5 biomarkers for distinguishing Constipation_F from Constipation_NF, Constipation_F from C, and Constipation_NF from C, respectively. Functional analysis revealed that pathways (P381-PWY and PWY-5507) related to vitamin B12 synthesis were reduced in Constipation_F, which aligns with the decreased abundances of vitamin-B12-producing Actinomyces and Akkermansia in this group. Our study unveils substantial differences in gut microbiota between constipated elders with frailty and those without, underscoring the diagnostic and therapeutic potential of genera involved in vitamin B12 synthesis.

KW - Microbial metabolism

KW - Microbiology

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DO - 10.1016/j.isci.2024.110403

M3 - Article

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VL - 27

JO - iScience

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ER -

Diminished representation of vitamin-B12-producing bacteria in constipated elders with frailty

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