@inproceedings{47a0c976cd524a278c72e3b154fe7290,
title = "Differential proteome of the striatum from A30P α-Synuclein transgenic mouse model of parkinson's disease",
abstract = "Parkinson's disease (PD) is a multifactorial, neurodegenerative disease where etiopathogenesis are not fully understood. Mutations in α-Synuclein (α-Syn) were the first genetic defect linked to PD. They are deposited in Lewy bodies (LBs) characteristic for PD. Some experiments had showed that A30P mutant a-Syn have high toxicity than wide-type α-Syn. Here we used A30Pα-Syn transgenic mice model to analysed proteome changes of the striatum 11 months after the birth. Striata were removed and after digesting the proteins we used isotope labelling method to mark different group of peptides. Strong-cation exchange (SCX) liquid chromatography (LC) was integrated with peptide separation as the first dimension of the two-dimensional LC tandem mass spectrometry workflow. In this work, electrospray ionization (ESI) quadrupole time-of-flight (QTOF) mass spectrometer was explored as a means of detecting the Ms/Ms spectrogram. Agilent Spectrum Mill software was used to analysed the results. A total of 660 proteins were quantified. 280 proteins were down-regulated and 77 proteins were up-regulated.",
keywords = "LC-Ms/Ms, Parkinson's disease, differential proteome analysis, α-Synuclein",
author = "Linna Guo and Jinyan Duan and Yan Xiong and Hong Qing and Yulin Deng",
year = "2013",
doi = "10.1109/ICCME.2013.6548335",
language = "English",
isbn = "9781467329699",
series = "2013 ICME International Conference on Complex Medical Engineering, CME 2013",
pages = "674--677",
booktitle = "2013 ICME International Conference on Complex Medical Engineering, CME 2013",
note = "2013 7th ICME International Conference on Complex Medical Engineering, CME 2013 ; Conference date: 25-05-2013 Through 28-05-2013",
}