Determination of the Abundance Proteins of Adipose Synthesis and β-Oxidation Using 18O Isotope Labeled and QconCAT Protein Combined with Multiple Reaction Monitoring Mass Spectrometry

Chi Zhang; Jie Hong; Yong Qian Zhang; Yu Lin Deng

Determination of the Abundance Proteins of Adipose Synthesis and β-Oxidation Using ¹⁸O Isotope Labeled and QconCAT Protein Combined with Multiple Reaction Monitoring Mass Spectrometry

Chi Zhang, Jie Hong, Yong Qian Zhang^*, Yu Lin Deng

^*Corresponding author for this work

School of Medical and Technology

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

A novel method using QconCAT protein combined with ¹⁸O-labeling technique and multiple reaction monitoring mass spectrometry was established to quantify proteins involved in the pathway of adipose synthesis and β-oxidation. Firstly, the QconCAT proteins were constructed, and expressed in Escherichia coli. The digestion and the ¹⁸O labeling efficiency were optimized. Secondly, the novel method of quantifying adipose synthesis and β-oxidation was developed. Lastly, target proteins of obesity and control groups were quantified by MRM. The results showed that the abundance of the enoyl ACP reductase and malonyl-COA ACP transferase increased significantly in the obesity group with P<0.05. It is consistent to previous reports and means the method had its reliability to analyze the biological sample.

Original language	English
Pages (from-to)	29-34
Number of pages	6
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	37
Publication status	Published - 1 Jun 2017

Keywords

Concatamers of Q peptides
Multiple reaction monitoring
O labeling

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Determination of the Abundance Proteins of Adipose Synthesis and β-Oxidation Using 18O Isotope Labeled and QconCAT Protein Combined with Multiple Reaction Monitoring Mass Spectrometry",

abstract = "A novel method using QconCAT protein combined with 18O-labeling technique and multiple reaction monitoring mass spectrometry was established to quantify proteins involved in the pathway of adipose synthesis and β-oxidation. Firstly, the QconCAT proteins were constructed, and expressed in Escherichia coli. The digestion and the 18O labeling efficiency were optimized. Secondly, the novel method of quantifying adipose synthesis and β-oxidation was developed. Lastly, target proteins of obesity and control groups were quantified by MRM. The results showed that the abundance of the enoyl ACP reductase and malonyl-COA ACP transferase increased significantly in the obesity group with P<0.05. It is consistent to previous reports and means the method had its reliability to analyze the biological sample.",

keywords = "Concatamers of Q peptides, Multiple reaction monitoring, O labeling",

author = "Chi Zhang and Jie Hong and Zhang, {Yong Qian} and Deng, {Yu Lin}",

year = "2017",

month = jun,

day = "1",

language = "English",

volume = "37",

pages = "29--34",

journal = "Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology",

issn = "1001-0645",

publisher = "Beijing Institute of Technology",

}

Determination of the Abundance Proteins of Adipose Synthesis and β-Oxidation Using ¹⁸O Isotope Labeled and QconCAT Protein Combined with Multiple Reaction Monitoring Mass Spectrometry. / Zhang, Chi; Hong, Jie; Zhang, Yong Qian et al.
In: Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology, Vol. 37, 01.06.2017, p. 29-34.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Determination of the Abundance Proteins of Adipose Synthesis and β-Oxidation Using 18O Isotope Labeled and QconCAT Protein Combined with Multiple Reaction Monitoring Mass Spectrometry

AU - Zhang, Chi

AU - Hong, Jie

AU - Zhang, Yong Qian

AU - Deng, Yu Lin

PY - 2017/6/1

Y1 - 2017/6/1

N2 - A novel method using QconCAT protein combined with 18O-labeling technique and multiple reaction monitoring mass spectrometry was established to quantify proteins involved in the pathway of adipose synthesis and β-oxidation. Firstly, the QconCAT proteins were constructed, and expressed in Escherichia coli. The digestion and the 18O labeling efficiency were optimized. Secondly, the novel method of quantifying adipose synthesis and β-oxidation was developed. Lastly, target proteins of obesity and control groups were quantified by MRM. The results showed that the abundance of the enoyl ACP reductase and malonyl-COA ACP transferase increased significantly in the obesity group with P<0.05. It is consistent to previous reports and means the method had its reliability to analyze the biological sample.

AB - A novel method using QconCAT protein combined with 18O-labeling technique and multiple reaction monitoring mass spectrometry was established to quantify proteins involved in the pathway of adipose synthesis and β-oxidation. Firstly, the QconCAT proteins were constructed, and expressed in Escherichia coli. The digestion and the 18O labeling efficiency were optimized. Secondly, the novel method of quantifying adipose synthesis and β-oxidation was developed. Lastly, target proteins of obesity and control groups were quantified by MRM. The results showed that the abundance of the enoyl ACP reductase and malonyl-COA ACP transferase increased significantly in the obesity group with P<0.05. It is consistent to previous reports and means the method had its reliability to analyze the biological sample.

KW - Concatamers of Q peptides

KW - Multiple reaction monitoring

KW - O labeling

UR - http://www.scopus.com/inward/record.url?scp=85033672157&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85033672157

SN - 1001-0645

VL - 37

SP - 29

EP - 34

JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

ER -

Determination of the Abundance Proteins of Adipose Synthesis and β-Oxidation Using ¹⁸O Isotope Labeled and QconCAT Protein Combined with Multiple Reaction Monitoring Mass Spectrometry

Abstract

Keywords

UN SDGs

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