Determination of S-catechol-O-methyltransferase activity in rat tissues using high-performance liquid chromatography with electrochemical detection

Yang Lina; Li Yujuan; Xiao Shengyuan; Deng Yulin

doi:10.1109/ICBBE.2009.5162625

Determination of S-catechol-O-methyltransferase activity in rat tissues using high-performance liquid chromatography with electrochemical detection

Yang Lina^*, Li Yujuan, Xiao Shengyuan, Deng Yulin

^*Corresponding author for this work

School of Medical and Technology

Beijing Institute of Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

A high-performance liquid chromatography with electrochemical detection (HPLC-ECD) method was developed to determine the activity of S-catechol-O-methyltransferase (COMT) in rat tissues including the liver, kidney, lung ,spleen and brain. The 3,4-dihydroxybenzoic acid was used as the substrate for SCOMT in rat tissues. After incubated with different tissues, the product 4-hydroxy-3-methoxybenzoic acid was formed from the substrate and separated to measure the COMT activity. The analysis was performed on a Agilent C₈ column (5μm, 150x4.6mm) at 550mV potential. The mobile phase was a mixture of methanol and 0.1MNa₂HPO₄(pH=3.3) containing 0.13mM ethylenediamine tetraacetic acid (v/v, 14/86) with a flow rate of 1ml/min. This approach was used to study S-COMT activity in vitro from various tissues.

Original language	English
Title of host publication	3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
DOIs	https://doi.org/10.1109/ICBBE.2009.5162625
Publication status	Published - 2009
Event	3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 - Beijing, China Duration: 11 Jun 2009 → 13 Jun 2009

Publication series

Name	3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009

Conference

Conference	3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
Country/Territory	China
City	Beijing
Period	11/06/09 → 13/06/09

Keywords

4-hydroxy-3-methoxybenzoic acid
Catechol-O-methyltransferase
HPLC-ECD
Rat tissues

Access to Document

10.1109/ICBBE.2009.5162625

Cite this

Lina, Y., Yujuan, L., Shengyuan, X., & Yulin, D. (2009). Determination of S-catechol-O-methyltransferase activity in rat tissues using high-performance liquid chromatography with electrochemical detection. In 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 Article 5162625 (3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009). https://doi.org/10.1109/ICBBE.2009.5162625

Lina, Yang ; Yujuan, Li ; Shengyuan, Xiao et al. / Determination of S-catechol-O-methyltransferase activity in rat tissues using high-performance liquid chromatography with electrochemical detection. 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009. 2009. (3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009).

@inproceedings{dae63c4976234f53812fd5c403d30bb7,

title = "Determination of S-catechol-O-methyltransferase activity in rat tissues using high-performance liquid chromatography with electrochemical detection",

abstract = "A high-performance liquid chromatography with electrochemical detection (HPLC-ECD) method was developed to determine the activity of S-catechol-O-methyltransferase (COMT) in rat tissues including the liver, kidney, lung ,spleen and brain. The 3,4-dihydroxybenzoic acid was used as the substrate for SCOMT in rat tissues. After incubated with different tissues, the product 4-hydroxy-3-methoxybenzoic acid was formed from the substrate and separated to measure the COMT activity. The analysis was performed on a Agilent C8 column (5μm, 150x4.6mm) at 550mV potential. The mobile phase was a mixture of methanol and 0.1MNa2HPO4(pH=3.3) containing 0.13mM ethylenediamine tetraacetic acid (v/v, 14/86) with a flow rate of 1ml/min. This approach was used to study S-COMT activity in vitro from various tissues.",

keywords = "4-hydroxy-3-methoxybenzoic acid, Catechol-O-methyltransferase, HPLC-ECD, Rat tissues",

author = "Yang Lina and Li Yujuan and Xiao Shengyuan and Deng Yulin",

year = "2009",

doi = "10.1109/ICBBE.2009.5162625",

language = "English",

isbn = "9781424429028",

series = "3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009",

booktitle = "3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009",

note = "3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 ; Conference date: 11-06-2009 Through 13-06-2009",

}

Lina, Y, Yujuan, L, Shengyuan, X & Yulin, D 2009, Determination of S-catechol-O-methyltransferase activity in rat tissues using high-performance liquid chromatography with electrochemical detection. in 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009., 5162625, 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009, 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009, Beijing, China, 11/06/09. https://doi.org/10.1109/ICBBE.2009.5162625

Determination of S-catechol-O-methyltransferase activity in rat tissues using high-performance liquid chromatography with electrochemical detection. / Lina, Yang; Yujuan, Li; Shengyuan, Xiao et al.
3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009. 2009. 5162625 (3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Determination of S-catechol-O-methyltransferase activity in rat tissues using high-performance liquid chromatography with electrochemical detection

AU - Lina, Yang

AU - Yujuan, Li

AU - Shengyuan, Xiao

AU - Yulin, Deng

PY - 2009

Y1 - 2009

N2 - A high-performance liquid chromatography with electrochemical detection (HPLC-ECD) method was developed to determine the activity of S-catechol-O-methyltransferase (COMT) in rat tissues including the liver, kidney, lung ,spleen and brain. The 3,4-dihydroxybenzoic acid was used as the substrate for SCOMT in rat tissues. After incubated with different tissues, the product 4-hydroxy-3-methoxybenzoic acid was formed from the substrate and separated to measure the COMT activity. The analysis was performed on a Agilent C8 column (5μm, 150x4.6mm) at 550mV potential. The mobile phase was a mixture of methanol and 0.1MNa2HPO4(pH=3.3) containing 0.13mM ethylenediamine tetraacetic acid (v/v, 14/86) with a flow rate of 1ml/min. This approach was used to study S-COMT activity in vitro from various tissues.

AB - A high-performance liquid chromatography with electrochemical detection (HPLC-ECD) method was developed to determine the activity of S-catechol-O-methyltransferase (COMT) in rat tissues including the liver, kidney, lung ,spleen and brain. The 3,4-dihydroxybenzoic acid was used as the substrate for SCOMT in rat tissues. After incubated with different tissues, the product 4-hydroxy-3-methoxybenzoic acid was formed from the substrate and separated to measure the COMT activity. The analysis was performed on a Agilent C8 column (5μm, 150x4.6mm) at 550mV potential. The mobile phase was a mixture of methanol and 0.1MNa2HPO4(pH=3.3) containing 0.13mM ethylenediamine tetraacetic acid (v/v, 14/86) with a flow rate of 1ml/min. This approach was used to study S-COMT activity in vitro from various tissues.

KW - 4-hydroxy-3-methoxybenzoic acid

KW - Catechol-O-methyltransferase

KW - HPLC-ECD

KW - Rat tissues

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SN - 9781424429028

T3 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009

BT - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009

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Y2 - 11 June 2009 through 13 June 2009

ER -

Lina Y, Yujuan L, Shengyuan X, Yulin D. Determination of S-catechol-O-methyltransferase activity in rat tissues using high-performance liquid chromatography with electrochemical detection. In 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009. 2009. 5162625. (3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009). doi: 10.1109/ICBBE.2009.5162625

Determination of S-catechol-O-methyltransferase activity in rat tissues using high-performance liquid chromatography with electrochemical detection

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