TY - GEN
T1 - Determination of monoamine oxidase activity by capillary electrophoresis method
AU - Peixiang, Ma
AU - Shanshan, Wang
AU - Feng, Qu
AU - Yulin, Deng
PY - 2007
Y1 - 2007
N2 - Amethod for the rapid determination of Monoamine Oxidase (MAO) activity by capillary electrophoresis (CE) has been established. The effect factors including the pH, the concentration of electroosmotic flow modifier (OFM), the salt concentration of running buffer were investigated in detail. The analysis was performed in an uncoated fused-silica capillary with 50 μm id and total length of 70 cm (40 cm to the detector) under the applied voltage of 15KV with a running buffer of 50 mmol/L phosphate buffer saline (PBS) and 0.5 mmol/L tetradecyltrimethyl ammonium bromide (TTAB) (pH 10.5), detected by UV detector at 214nm. The linear range of 4-Hydroxyyquinoline, which is the product of the MAO deaminated Kynuramine, between peak area and concentrationwas was from 10 μmol/L to 1 000 μmol/L, with correlation coefficient of 0. 9997. The precision of the method was below 8.5% (n =5). The limit of detection was 2 μmol/L (S /N = 3).
AB - Amethod for the rapid determination of Monoamine Oxidase (MAO) activity by capillary electrophoresis (CE) has been established. The effect factors including the pH, the concentration of electroosmotic flow modifier (OFM), the salt concentration of running buffer were investigated in detail. The analysis was performed in an uncoated fused-silica capillary with 50 μm id and total length of 70 cm (40 cm to the detector) under the applied voltage of 15KV with a running buffer of 50 mmol/L phosphate buffer saline (PBS) and 0.5 mmol/L tetradecyltrimethyl ammonium bromide (TTAB) (pH 10.5), detected by UV detector at 214nm. The linear range of 4-Hydroxyyquinoline, which is the product of the MAO deaminated Kynuramine, between peak area and concentrationwas was from 10 μmol/L to 1 000 μmol/L, with correlation coefficient of 0. 9997. The precision of the method was below 8.5% (n =5). The limit of detection was 2 μmol/L (S /N = 3).
UR - http://www.scopus.com/inward/record.url?scp=48149091037&partnerID=8YFLogxK
U2 - 10.1109/ICCME.2007.4382056
DO - 10.1109/ICCME.2007.4382056
M3 - Conference contribution
AN - SCOPUS:48149091037
SN - 1424410789
SN - 9781424410781
T3 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
SP - 1792
EP - 1795
BT - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
T2 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Y2 - 23 May 2007 through 27 May 2007
ER -
