Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation

Yoko Yamanishi; Shinya Sakuma; Tomohiro Iyanagi; Fumihito Arai; Tatsuo Arai; Akiyuki Hasegawa; Tamio Tanikawa; Akihiko Ichikawa; Osamu Satoh; Akihiro Nakayama; Hiroshi Aso; Mitsuhiro Goto; Seiya Takahashi; Kazutsugu Matsukawa

doi:10.20965/jrm.2010.p0371

Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation

Yoko Yamanishi^*, Shinya Sakuma, Tomohiro Iyanagi, Fumihito Arai, Tatsuo Arai, Akiyuki Hasegawa, Tamio Tanikawa, Akihiko Ichikawa, Osamu Satoh, Akihiro Nakayama, Hiroshi Aso, Mitsuhiro Goto, Seiya Takahashi, Kazutsugu Matsukawa

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and (4) they are fused by an electrical field. For the current study, the all-in-one unified microfluidic chip was designed to execute (1) cutting, (2) sorting, and (3) coupling parts continuously for this process. Basic functions of these parts as well as fusion part are verified independently. Then, all-inone unified microfluidic chip was successfully designed and fabricated.

Original language	English
Pages (from-to)	371-379
Number of pages	9
Journal	Journal of Robotics and Mechatronics
Volume	22
Issue number	3
DOIs	https://doi.org/10.20965/jrm.2010.p0371
Publication status	Published - 2010
Externally published	Yes

Keywords

biochip
cell manipulation
cloning
microTAS
photolithography

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10.20965/jrm.2010.p0371

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Yamanishi, Y., Sakuma, S., Iyanagi, T., Arai, F., Arai, T., Hasegawa, A., Tanikawa, T., Ichikawa, A., Satoh, O., Nakayama, A., Aso, H., Goto, M., Takahashi, S., & Matsukawa, K. (2010). Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation. Journal of Robotics and Mechatronics, 22(3), 371-379. https://doi.org/10.20965/jrm.2010.p0371

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title = "Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation",

abstract = "We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and (4) they are fused by an electrical field. For the current study, the all-in-one unified microfluidic chip was designed to execute (1) cutting, (2) sorting, and (3) coupling parts continuously for this process. Basic functions of these parts as well as fusion part are verified independently. Then, all-inone unified microfluidic chip was successfully designed and fabricated.",

keywords = "biochip, cell manipulation, cloning, microTAS, photolithography",

author = "Yoko Yamanishi and Shinya Sakuma and Tomohiro Iyanagi and Fumihito Arai and Tatsuo Arai and Akiyuki Hasegawa and Tamio Tanikawa and Akihiko Ichikawa and Osamu Satoh and Akihiro Nakayama and Hiroshi Aso and Mitsuhiro Goto and Seiya Takahashi and Kazutsugu Matsukawa",

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Yamanishi, Y, Sakuma, S, Iyanagi, T, Arai, F, Arai, T, Hasegawa, A, Tanikawa, T, Ichikawa, A, Satoh, O, Nakayama, A, Aso, H, Goto, M, Takahashi, S & Matsukawa, K 2010, 'Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation', Journal of Robotics and Mechatronics, vol. 22, no. 3, pp. 371-379. https://doi.org/10.20965/jrm.2010.p0371

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AU - Yamanishi, Yoko

AU - Sakuma, Shinya

AU - Iyanagi, Tomohiro

AU - Arai, Fumihito

AU - Arai, Tatsuo

AU - Hasegawa, Akiyuki

AU - Tanikawa, Tamio

AU - Ichikawa, Akihiko

AU - Satoh, Osamu

AU - Nakayama, Akihiro

AU - Aso, Hiroshi

AU - Goto, Mitsuhiro

AU - Takahashi, Seiya

AU - Matsukawa, Kazutsugu

PY - 2010

Y1 - 2010

N2 - We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and (4) they are fused by an electrical field. For the current study, the all-in-one unified microfluidic chip was designed to execute (1) cutting, (2) sorting, and (3) coupling parts continuously for this process. Basic functions of these parts as well as fusion part are verified independently. Then, all-inone unified microfluidic chip was successfully designed and fabricated.

AB - We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and (4) they are fused by an electrical field. For the current study, the all-in-one unified microfluidic chip was designed to execute (1) cutting, (2) sorting, and (3) coupling parts continuously for this process. Basic functions of these parts as well as fusion part are verified independently. Then, all-inone unified microfluidic chip was successfully designed and fabricated.

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KW - cell manipulation

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KW - microTAS

KW - photolithography

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JF - Journal of Robotics and Mechatronics

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ER -

Design and Fabrication of All-in-One Unified Microfluidic Chip for Automation of Embryonic Cell Manipulation

Abstract

Keywords

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