Guo, D., Liu, H., Gao, G., Liu, Y., Zhuang, Y., Yang, F., Wang, K., Zhou, T., Qin, D., Hong, L., Li, J., Xu, K., & Li, Y. X. (2017). Creating a patient carried Men1 gene point mutation on wild type iPSCs locus mediated by CRISPR/Cas9 and ssODN. Stem Cell Research, 18, 67-69. https://doi.org/10.1016/j.scr.2016.12.007
Guo, Dongsheng ; Liu, Haikun ; Gao, Ge et al. / Creating a patient carried Men1 gene point mutation on wild type iPSCs locus mediated by CRISPR/Cas9 and ssODN. In: Stem Cell Research. 2017 ; Vol. 18. pp. 67-69.
@article{60aeb15006804057ae0f49cefe34f8af,
title = "Creating a patient carried Men1 gene point mutation on wild type iPSCs locus mediated by CRISPR/Cas9 and ssODN",
abstract = "A patient specific point mutation (c.1288G > T) of Men1 gene was introduced into wide type iPSC line with CRISPR/Cas9 and single-stranded donor oligonucleotides carrying the mutation. The mutated iPSC line has a heterozygous c.1288G > T mutation on exon-9 of Men1 that was confirmed by sequencing analysis. The karyotype of this line was normal and the pluripotency was demonstrated by its ability to differentiate into three germ layers. These artificially created Men1 mutation in wild type iPSC line will help to dissect out the molecular basis of two patients carried the same mutation from one family who were differentially represented hypoglycemia.",
author = "Dongsheng Guo and Haikun Liu and Ge Gao and Yanli Liu and Yuanqi Zhuang and Fan Yang and Kepin Wang and Tiancheng Zhou and Dajiang Qin and Liangqing Hong and Jialiang Li and Kecheng Xu and Li, {Yin xiong}",
note = "Publisher Copyright: {\textcopyright} 2016 The Authors",
year = "2017",
month = jan,
doi = "10.1016/j.scr.2016.12.007",
language = "English",
volume = "18",
pages = "67--69",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier B.V.",
}
Guo, D, Liu, H, Gao, G, Liu, Y, Zhuang, Y, Yang, F, Wang, K, Zhou, T, Qin, D, Hong, L, Li, J, Xu, K & Li, YX 2017, 'Creating a patient carried Men1 gene point mutation on wild type iPSCs locus mediated by CRISPR/Cas9 and ssODN', Stem Cell Research, vol. 18, pp. 67-69. https://doi.org/10.1016/j.scr.2016.12.007
Creating a patient carried Men1 gene point mutation on wild type iPSCs locus mediated by CRISPR/Cas9 and ssODN. / Guo, Dongsheng; Liu, Haikun
; Gao, Ge et al.
In:
Stem Cell Research, Vol. 18, 01.2017, p. 67-69.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Creating a patient carried Men1 gene point mutation on wild type iPSCs locus mediated by CRISPR/Cas9 and ssODN
AU - Guo, Dongsheng
AU - Liu, Haikun
AU - Gao, Ge
AU - Liu, Yanli
AU - Zhuang, Yuanqi
AU - Yang, Fan
AU - Wang, Kepin
AU - Zhou, Tiancheng
AU - Qin, Dajiang
AU - Hong, Liangqing
AU - Li, Jialiang
AU - Xu, Kecheng
AU - Li, Yin xiong
N1 - Publisher Copyright:
© 2016 The Authors
PY - 2017/1
Y1 - 2017/1
N2 - A patient specific point mutation (c.1288G > T) of Men1 gene was introduced into wide type iPSC line with CRISPR/Cas9 and single-stranded donor oligonucleotides carrying the mutation. The mutated iPSC line has a heterozygous c.1288G > T mutation on exon-9 of Men1 that was confirmed by sequencing analysis. The karyotype of this line was normal and the pluripotency was demonstrated by its ability to differentiate into three germ layers. These artificially created Men1 mutation in wild type iPSC line will help to dissect out the molecular basis of two patients carried the same mutation from one family who were differentially represented hypoglycemia.
AB - A patient specific point mutation (c.1288G > T) of Men1 gene was introduced into wide type iPSC line with CRISPR/Cas9 and single-stranded donor oligonucleotides carrying the mutation. The mutated iPSC line has a heterozygous c.1288G > T mutation on exon-9 of Men1 that was confirmed by sequencing analysis. The karyotype of this line was normal and the pluripotency was demonstrated by its ability to differentiate into three germ layers. These artificially created Men1 mutation in wild type iPSC line will help to dissect out the molecular basis of two patients carried the same mutation from one family who were differentially represented hypoglycemia.
UR - http://www.scopus.com/inward/record.url?scp=85008162773&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2016.12.007
DO - 10.1016/j.scr.2016.12.007
M3 - Article
C2 - 28395809
AN - SCOPUS:85008162773
SN - 1873-5061
VL - 18
SP - 67
EP - 69
JO - Stem Cell Research
JF - Stem Cell Research
ER -
Guo D, Liu H, Gao G, Liu Y, Zhuang Y, Yang F et al. Creating a patient carried Men1 gene point mutation on wild type iPSCs locus mediated by CRISPR/Cas9 and ssODN. Stem Cell Research. 2017 Jan;18:67-69. doi: 10.1016/j.scr.2016.12.007