CO2-switchable response of protein microtubules: Behaviour and mechanism

Guang Yang, Rongting Hu, Hong Ming Ding*, Zdravko Kochovski, Shilin Mei, Yan Lu, Yu Qiang Ma, Guosong Chen, Ming Jiang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Recently, we proposed a small molecular "inducing ligand" strategy to assemble proteins into highly-ordered structures via dual non-covalent interactions, i.e. carbohydrate-protein interaction and dimerization of Rhodamine B. Using this approach, artificial protein microtubules were successfully constructed. In this study, we find that these microtubules exhibit a perfect CO2 responsiveness; assembly and disassembly of these microtubules were nicely controlled by the alternative passage of CO2 and N2. Upon the injection of CO2, a negative net-charged SBA turns into a neutral or positive net-charged SBA, which elongated, to some extent, the effective distance between SBA and Rhodamine B, resulting in the disassociation of the Rhodamine B dimer. Further experimental and simulation results reveal that the CO2-responsive mechanism differs from that of solubility change of the previously reported CO2-responsive synthetic materials.

Original languageEnglish
Pages (from-to)1642-1646
Number of pages5
JournalMaterials Chemistry Frontiers
Volume2
Issue number9
DOIs
Publication statusPublished - Sept 2018
Externally publishedYes

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