Comprehensive proteome analysis of human smooth muscle cells by 2D-HPLC-ESI-MS/MS

Yongqian Zhang; Chengjun Lai; Yong Zhu; Yulin Deng

doi:10.1109/icbbe.2011.5780052

Comprehensive proteome analysis of human smooth muscle cells by 2D-HPLC-ESI-MS/MS

Yongqian Zhang, Chengjun Lai, Yong Zhu, Yulin Deng^*

^*Corresponding author for this work

School of Medical and Technology

Beijing Institute of Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The proteome of smooth muscle cells of human umbilical cord by offline SCX and LC-ESI-MS/MS are investigated. The MS/MS data generated by data-dependent acquisition via the ion trap was searched by the Mascot. 383 proteins were identified and characterized based on annotated component, function, and biological process by Gene Ontology (GO).

Original language	English
Title of host publication	5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011
DOIs	https://doi.org/10.1109/icbbe.2011.5780052
Publication status	Published - 2011
Event	5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011 - Wuhan, China Duration: 10 May 2011 → 12 May 2011

Publication series

Name	5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011

Conference

Conference	5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011
Country/Territory	China
City	Wuhan
Period	10/05/11 → 12/05/11

Keywords

2DLC-ESI-MS-MS
SCX
Shotgun

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10.1109/icbbe.2011.5780052

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Zhang, Y., Lai, C., Zhu, Y., & Deng, Y. (2011). Comprehensive proteome analysis of human smooth muscle cells by 2D-HPLC-ESI-MS/MS. In 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011 Article 5780052 (5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011). https://doi.org/10.1109/icbbe.2011.5780052

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title = "Comprehensive proteome analysis of human smooth muscle cells by 2D-HPLC-ESI-MS/MS",

abstract = "The proteome of smooth muscle cells of human umbilical cord by offline SCX and LC-ESI-MS/MS are investigated. The MS/MS data generated by data-dependent acquisition via the ion trap was searched by the Mascot. 383 proteins were identified and characterized based on annotated component, function, and biological process by Gene Ontology (GO).",

keywords = "2DLC-ESI-MS-MS, SCX, Shotgun",

author = "Yongqian Zhang and Chengjun Lai and Yong Zhu and Yulin Deng",

year = "2011",

doi = "10.1109/icbbe.2011.5780052",

language = "English",

isbn = "9781424450893",

series = "5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011",

booktitle = "5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011",

note = "5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011 ; Conference date: 10-05-2011 Through 12-05-2011",

}

Zhang, Y, Lai, C, Zhu, Y & Deng, Y 2011, Comprehensive proteome analysis of human smooth muscle cells by 2D-HPLC-ESI-MS/MS. in 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011., 5780052, 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011, 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011, Wuhan, China, 10/05/11. https://doi.org/10.1109/icbbe.2011.5780052

Comprehensive proteome analysis of human smooth muscle cells by 2D-HPLC-ESI-MS/MS. / Zhang, Yongqian; Lai, Chengjun; Zhu, Yong et al.
5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011. 2011. 5780052 (5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Comprehensive proteome analysis of human smooth muscle cells by 2D-HPLC-ESI-MS/MS

AU - Zhang, Yongqian

AU - Lai, Chengjun

AU - Zhu, Yong

AU - Deng, Yulin

PY - 2011

Y1 - 2011

N2 - The proteome of smooth muscle cells of human umbilical cord by offline SCX and LC-ESI-MS/MS are investigated. The MS/MS data generated by data-dependent acquisition via the ion trap was searched by the Mascot. 383 proteins were identified and characterized based on annotated component, function, and biological process by Gene Ontology (GO).

AB - The proteome of smooth muscle cells of human umbilical cord by offline SCX and LC-ESI-MS/MS are investigated. The MS/MS data generated by data-dependent acquisition via the ion trap was searched by the Mascot. 383 proteins were identified and characterized based on annotated component, function, and biological process by Gene Ontology (GO).

KW - 2DLC-ESI-MS-MS

KW - SCX

KW - Shotgun

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U2 - 10.1109/icbbe.2011.5780052

DO - 10.1109/icbbe.2011.5780052

M3 - Conference contribution

AN - SCOPUS:79960129592

SN - 9781424450893

T3 - 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011

BT - 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011

T2 - 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011

Y2 - 10 May 2011 through 12 May 2011

ER -

Comprehensive proteome analysis of human smooth muscle cells by 2D-HPLC-ESI-MS/MS

Abstract

Publication series

Conference

Keywords

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