Li, L., Lu, L., Ren, Y., Tang, G., Zhao, Y., Cai, X., Shi, Z., Ding, H., Liu, C., Cheng, D., Xie, Y., Wang, H., Fu, X., Yin, L., Luo, M., & Sheng, X. (2022). Colocalized, bidirectional optogenetic modulations in freely behaving mice with a wireless dual-color optoelectronic probe. Nature Communications, 13(1), Article 839. https://doi.org/10.1038/s41467-022-28539-7
Li, Lizhu ; Lu, Lihui ; Ren, Yuqi et al. / Colocalized, bidirectional optogenetic modulations in freely behaving mice with a wireless dual-color optoelectronic probe. In: Nature Communications. 2022 ; Vol. 13, No. 1.
@article{fea3e2a885194ddabb21e2d14bd26357,
title = "Colocalized, bidirectional optogenetic modulations in freely behaving mice with a wireless dual-color optoelectronic probe",
abstract = "Optogenetic methods provide efficient cell-specific modulations, and the ability of simultaneous neural activation and inhibition in the same brain region of freely moving animals is highly desirable. Here we report bidirectional neuronal activity manipulation accomplished by a wireless, dual-color optogenetic probe in synergy with the co-expression of two spectrally distinct opsins (ChrimsonR and stGtACR2) in a rodent model. The flexible probe comprises vertically assembled, thin-film microscale light-emitting diodes with a lateral dimension of 125 × 180 µm2, showing colocalized red and blue emissions and enabling chronic in vivo operations with desirable biocompatibilities. Red or blue irradiations deterministically evoke or silence neurons co-expressing the two opsins. The probe interferes with dopaminergic neurons in the ventral tegmental area of mice, increasing or decreasing dopamine levels. Such bidirectional regulations further generate rewarding and aversive behaviors and interrogate social interactions among multiple mice. These technologies create numerous opportunities and implications for brain research.",
author = "Lizhu Li and Lihui Lu and Yuqi Ren and Guo Tang and Yu Zhao and Xue Cai and Zhao Shi and He Ding and Changbo Liu and Dali Cheng and Yang Xie and Huachun Wang and Xin Fu and Lan Yin and Minmin Luo and Xing Sheng",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
doi = "10.1038/s41467-022-28539-7",
language = "English",
volume = "13",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}
Li, L, Lu, L, Ren, Y, Tang, G, Zhao, Y, Cai, X, Shi, Z, Ding, H, Liu, C, Cheng, D, Xie, Y, Wang, H, Fu, X, Yin, L, Luo, M & Sheng, X 2022, 'Colocalized, bidirectional optogenetic modulations in freely behaving mice with a wireless dual-color optoelectronic probe', Nature Communications, vol. 13, no. 1, 839. https://doi.org/10.1038/s41467-022-28539-7
Colocalized, bidirectional optogenetic modulations in freely behaving mice with a wireless dual-color optoelectronic probe. / Li, Lizhu; Lu, Lihui; Ren, Yuqi et al.
In:
Nature Communications, Vol. 13, No. 1, 839, 12.2022.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Colocalized, bidirectional optogenetic modulations in freely behaving mice with a wireless dual-color optoelectronic probe
AU - Li, Lizhu
AU - Lu, Lihui
AU - Ren, Yuqi
AU - Tang, Guo
AU - Zhao, Yu
AU - Cai, Xue
AU - Shi, Zhao
AU - Ding, He
AU - Liu, Changbo
AU - Cheng, Dali
AU - Xie, Yang
AU - Wang, Huachun
AU - Fu, Xin
AU - Yin, Lan
AU - Luo, Minmin
AU - Sheng, Xing
N1 - Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Optogenetic methods provide efficient cell-specific modulations, and the ability of simultaneous neural activation and inhibition in the same brain region of freely moving animals is highly desirable. Here we report bidirectional neuronal activity manipulation accomplished by a wireless, dual-color optogenetic probe in synergy with the co-expression of two spectrally distinct opsins (ChrimsonR and stGtACR2) in a rodent model. The flexible probe comprises vertically assembled, thin-film microscale light-emitting diodes with a lateral dimension of 125 × 180 µm2, showing colocalized red and blue emissions and enabling chronic in vivo operations with desirable biocompatibilities. Red or blue irradiations deterministically evoke or silence neurons co-expressing the two opsins. The probe interferes with dopaminergic neurons in the ventral tegmental area of mice, increasing or decreasing dopamine levels. Such bidirectional regulations further generate rewarding and aversive behaviors and interrogate social interactions among multiple mice. These technologies create numerous opportunities and implications for brain research.
AB - Optogenetic methods provide efficient cell-specific modulations, and the ability of simultaneous neural activation and inhibition in the same brain region of freely moving animals is highly desirable. Here we report bidirectional neuronal activity manipulation accomplished by a wireless, dual-color optogenetic probe in synergy with the co-expression of two spectrally distinct opsins (ChrimsonR and stGtACR2) in a rodent model. The flexible probe comprises vertically assembled, thin-film microscale light-emitting diodes with a lateral dimension of 125 × 180 µm2, showing colocalized red and blue emissions and enabling chronic in vivo operations with desirable biocompatibilities. Red or blue irradiations deterministically evoke or silence neurons co-expressing the two opsins. The probe interferes with dopaminergic neurons in the ventral tegmental area of mice, increasing or decreasing dopamine levels. Such bidirectional regulations further generate rewarding and aversive behaviors and interrogate social interactions among multiple mice. These technologies create numerous opportunities and implications for brain research.
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U2 - 10.1038/s41467-022-28539-7
DO - 10.1038/s41467-022-28539-7
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SN - 2041-1723
VL - 13
JO - Nature Communications
JF - Nature Communications
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ER -
Li L, Lu L, Ren Y, Tang G, Zhao Y, Cai X et al. Colocalized, bidirectional optogenetic modulations in freely behaving mice with a wireless dual-color optoelectronic probe. Nature Communications. 2022 Dec;13(1):839. doi: 10.1038/s41467-022-28539-7