Characterization of the interaction between surfactants and enzymes by fluorescence probe

Jia Liu*, Jingang Shi, Jian Li, Xingzhong Yuan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

In order to investigate the mechanism of the different stimulatory effects of the biosurfactant rhamnolipid and the chemical surfactant Tween 80 on enzymatic hydrolysis of lignocellulose, the interaction between surfactants and enzymes was analyzed by the fluorescence probe method using pyrene as probe. Based on the evolution law of pyrene fluorescence spectroscopy in the " surfactants-enzymes" systems, the interaction relationship between surfactants and enzymes was analyzed and discussed in this paper. The results show that enzyme molecules bind with rhamnolipid molecules, participate in the formation of rhamnolipid micelles, and increase the inner hydrophobic polarity of micelles, but do not change the properties of rhamnolipid micelles above the CMC (Critical Micelle Concentration). Nevertheless, for Tween 80, enzyme molecules also participate in the forming of micelles, however, they exhibit a stronger interaction with enzymes above the CMC. Both rhamnolipid and Tween 80 bind more strongly with xylanase than cellulase. Considering also previous experimental results, it can be concluded that the interaction between surfactants and enzymes improve enzyme stability and activity, and, therefore, the efficiency of enzymatic hydrolysis of lignocellulose is enhanced. The findings further provide theoretical knowledge about the mechanism of the stimulative effects of surfactants on enzymatic hydrolysis of lignocellulose.

Original languageEnglish
Pages (from-to)360-365
Number of pages6
JournalEnzyme and Microbial Technology
Volume49
Issue number4
DOIs
Publication statusPublished - 10 Sept 2011
Externally publishedYes

Keywords

  • Cellulase
  • Fluorescence probe
  • Rhamnolipid
  • Tween 80
  • Xylanase

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Liu, J., Shi, J., Li, J., & Yuan, X. (2011). Characterization of the interaction between surfactants and enzymes by fluorescence probe. Enzyme and Microbial Technology, 49(4), 360-365. https://doi.org/10.1016/j.enzmictec.2011.06.014