Biotemplated synthesis of cold nanoparticle-bacteria cellulose nanofiber nanocomposites and their application in biosensing

Taiji Zhang*, Wei Wang, Dayong Zhang, Xinxiang Zhang, Ma Yurong, Yinglin Zhou, Limin Qi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

347 Citations (Scopus)

Abstract

Bacteria cellulose (BC) nanofibers are used as robust biotemplates for the facile fabrication of novel gold nanoparticle (NP)-bacteria cellulose nanofiber (Au-BC) nanocomposites via a one-step method. The BC nanofibers are uniformly coated with Au NPs in aqueous suspension using poly(ethyleneimine) (PEI) as the reducing and linking agent. With the addition of different halides, Au-BC nanocomposites with different Au shell thicknesses are formed, and a possible formation mechanism is proposed by taking into account the special role played by PEI. A novel H2O2 biosensor is constructed using the obtained Au-BC nanocomposites as excellent support for horseradish peroxidase (NRP) immobilization, which allows the detection of H2O2 with a detection limit lower than 1 μM. The Au-BC nanocomposites could be further used for the immobilization of many other enzymes, and thus, may find potential applications in bioelectroanalysis and bioelectrocatalysis.

Original languageEnglish
Pages (from-to)1152-1160
Number of pages9
JournalAdvanced Functional Materials
Volume20
Issue number7
DOIs
Publication statusPublished - 9 Apr 2010
Externally publishedYes

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