Biosynthesis of D-1, 2, 4-butanetriol from D-xylose by recombinant Escherichia coli

Pengfei Ma, Jian Meng, Jing Zhou, Haijun Gao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

1, 2, 4-Butanetriol (BT) is an important organic synthetic intermediate. In this study, the metabolic network of Escherichia coli was reconstructed by heterogeneously expressing a keto acid decarboxylase (mdlC) from Pseudomonas putida ATCC12633 and a D-xylose dehydrogenase (xdh) from Caulobacter crescentus CB15, and knocking out xylA, yjhH and yagE which were the genes of xylose utilization pathway and intermediary metabolite pathway for D-1, 2, 4-butanetriol synthesis. The recombinant strain could synthesize D-1, 2, 4-butanetriol directly using D-xylose as precursor. Culture conditions such as temperature, medium volume, pH of fermentation broth were investigated at the titer of D-1, 2, 4-butanetriol of 3.96 g·L-1 under suitable fermentation conditions. The relationship between glucose utilization and D-1, 2, 4-butanetriol synthesis was discussed. After modifying the phosphoenolpyruvate: sugar phosphotransferase system (PTS) by knocking out ptsG the reconstructed E.coli could utilize glucose and xylose simultaneously, leading to a higher D-1, 2, 4-butanetriol productivity.

Original languageEnglish
Pages (from-to)2620-2627
Number of pages8
JournalHuagong Xuebao/CIESC Journal
Volume66
Issue number7
DOIs
Publication statusPublished - 1 Jul 2015

Keywords

  • Biocatalysis
  • D-1, 2, 4-butanetriol
  • Metabolism
  • PTS system
  • Synthetic biology

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Ma, P., Meng, J., Zhou, J., & Gao, H. (2015). Biosynthesis of D-1, 2, 4-butanetriol from D-xylose by recombinant Escherichia coli. Huagong Xuebao/CIESC Journal, 66(7), 2620-2627. https://doi.org/10.11949/j.issn.0438-1157.20141703