TY - JOUR
T1 - Biosynthesis of D-1, 2, 4-butanetriol from D-xylose by recombinant Escherichia coli
AU - Ma, Pengfei
AU - Meng, Jian
AU - Zhou, Jing
AU - Gao, Haijun
N1 - Publisher Copyright:
©, 2015, Chemical Industry Press. All right reserved.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - 1, 2, 4-Butanetriol (BT) is an important organic synthetic intermediate. In this study, the metabolic network of Escherichia coli was reconstructed by heterogeneously expressing a keto acid decarboxylase (mdlC) from Pseudomonas putida ATCC12633 and a D-xylose dehydrogenase (xdh) from Caulobacter crescentus CB15, and knocking out xylA, yjhH and yagE which were the genes of xylose utilization pathway and intermediary metabolite pathway for D-1, 2, 4-butanetriol synthesis. The recombinant strain could synthesize D-1, 2, 4-butanetriol directly using D-xylose as precursor. Culture conditions such as temperature, medium volume, pH of fermentation broth were investigated at the titer of D-1, 2, 4-butanetriol of 3.96 g·L-1 under suitable fermentation conditions. The relationship between glucose utilization and D-1, 2, 4-butanetriol synthesis was discussed. After modifying the phosphoenolpyruvate: sugar phosphotransferase system (PTS) by knocking out ptsG the reconstructed E.coli could utilize glucose and xylose simultaneously, leading to a higher D-1, 2, 4-butanetriol productivity.
AB - 1, 2, 4-Butanetriol (BT) is an important organic synthetic intermediate. In this study, the metabolic network of Escherichia coli was reconstructed by heterogeneously expressing a keto acid decarboxylase (mdlC) from Pseudomonas putida ATCC12633 and a D-xylose dehydrogenase (xdh) from Caulobacter crescentus CB15, and knocking out xylA, yjhH and yagE which were the genes of xylose utilization pathway and intermediary metabolite pathway for D-1, 2, 4-butanetriol synthesis. The recombinant strain could synthesize D-1, 2, 4-butanetriol directly using D-xylose as precursor. Culture conditions such as temperature, medium volume, pH of fermentation broth were investigated at the titer of D-1, 2, 4-butanetriol of 3.96 g·L-1 under suitable fermentation conditions. The relationship between glucose utilization and D-1, 2, 4-butanetriol synthesis was discussed. After modifying the phosphoenolpyruvate: sugar phosphotransferase system (PTS) by knocking out ptsG the reconstructed E.coli could utilize glucose and xylose simultaneously, leading to a higher D-1, 2, 4-butanetriol productivity.
KW - Biocatalysis
KW - D-1, 2, 4-butanetriol
KW - Metabolism
KW - PTS system
KW - Synthetic biology
UR - http://www.scopus.com/inward/record.url?scp=85050578268&partnerID=8YFLogxK
U2 - 10.11949/j.issn.0438-1157.20141703
DO - 10.11949/j.issn.0438-1157.20141703
M3 - Article
AN - SCOPUS:85050578268
SN - 0438-1157
VL - 66
SP - 2620
EP - 2627
JO - Huagong Xuebao/CIESC Journal
JF - Huagong Xuebao/CIESC Journal
IS - 7
ER -