Automated biomanipulation to assemble cellular microstructure with railed multi-microrobotic system

Huaping Wang, Qing Shi, Qiang Huang, Tao Sun, Toshio Fukuda, Masahiro Nakajima, Masaru Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The fabrication of vessel-mimetic microchannel provides critical access to enable sufficient nutrient delivery for engineered composite tissues. This paper presents a novel method to engineer vascular-like microchannel by automated assembly of donut-shaped micromodules embedding fibroblast cells. A railed microrobotic system is set up with multi manipulators of 30 nm positioning resolution under an optical microscope. The coordinated manipulation among the micromanipulators is performed with newly designed concentric movement along the rail, which realized the arbitrary change of micromanipulator posture. With the novel developed image processing algorithm for the tracking of micromanipulator and micromodules, the automated bio-assembly of vascular-like microchannel is achieved which is free from the influence of the occlusion during contact manipulation. Experimental results showed that the pick-up success rate is around 98% and tracking algorithm is robust. Finally, we achieved automated assembly of microchannel at a speed of 6 micromodules/min, which is efficient enough to fabricate vessel-mimetic substitute with macro-significance dimension for biological application.

Original languageEnglish
Title of host publication2015 IEEE International Conference on Information and Automation, ICIA 2015 - In conjunction with 2015 IEEE International Conference on Automation and Logistics
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages743-748
Number of pages6
ISBN (Electronic)9781467391047
DOIs
Publication statusPublished - 28 Sept 2015
Event2015 IEEE International Conference on Information and Automation, ICIA 2015 - In conjunction with 2015 IEEE International Conference on Automation and Logistics - Yunnan, China
Duration: 8 Aug 201510 Aug 2015

Publication series

Name2015 IEEE International Conference on Information and Automation, ICIA 2015 - In conjunction with 2015 IEEE International Conference on Automation and Logistics

Conference

Conference2015 IEEE International Conference on Information and Automation, ICIA 2015 - In conjunction with 2015 IEEE International Conference on Automation and Logistics
Country/TerritoryChina
CityYunnan
Period8/08/1510/08/15

Keywords

  • Automated micromanipulation
  • bio-assembly
  • image processing
  • microrobotic
  • tissue engineering

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