TY - GEN
T1 - Assay for affinity of proteins onto model biomembrane
AU - Jiang, Jing
AU - Wang, Shanshan
AU - Ma, Peixiang
AU - Qu, Feng
AU - Luo, Aiqin
AU - Deng, Yulin
PY - 2007
Y1 - 2007
N2 - In this paper, immobilized artificial membrane (IAM) was selected as the model biomembrane, and packed into capillary column as stationary phase of liquid chromatography to screen the proteins having strong interaction with biomembrane rapidly. The affinity between IAM and ten common proteins (pepsin, bovine serum albumin, casein, transferring, myosin, insulin, hemoglobin, cytochrome C, tryp sin, lysozyme), whose pI ranged from 2.0-11.5, were analyzed in different pH mobile phase including acidic, neutral and alkaline buffer. The optimized condition was obtained for the affinity of IAM and proteins. The proteins having strong affinity with IAM were screened out under each pH mobile phase. It can provide the experimental foundation for proteins to be immobilized onto biomembrane to study the membrane related interaction, separation, protein bioactivity, and drug screening and so on.
AB - In this paper, immobilized artificial membrane (IAM) was selected as the model biomembrane, and packed into capillary column as stationary phase of liquid chromatography to screen the proteins having strong interaction with biomembrane rapidly. The affinity between IAM and ten common proteins (pepsin, bovine serum albumin, casein, transferring, myosin, insulin, hemoglobin, cytochrome C, tryp sin, lysozyme), whose pI ranged from 2.0-11.5, were analyzed in different pH mobile phase including acidic, neutral and alkaline buffer. The optimized condition was obtained for the affinity of IAM and proteins. The proteins having strong affinity with IAM were screened out under each pH mobile phase. It can provide the experimental foundation for proteins to be immobilized onto biomembrane to study the membrane related interaction, separation, protein bioactivity, and drug screening and so on.
UR - http://www.scopus.com/inward/record.url?scp=48149084414&partnerID=8YFLogxK
U2 - 10.1109/ICCME.2007.4382032
DO - 10.1109/ICCME.2007.4382032
M3 - Conference contribution
AN - SCOPUS:48149084414
SN - 1424410789
SN - 9781424410781
T3 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
SP - 1676
EP - 1680
BT - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
T2 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Y2 - 23 May 2007 through 27 May 2007
ER -
