Aptamer-based fluorometric lateral flow assay for creatine kinase MB

Jing Zhang; Xuefei Lv; Wei Feng; Xiaoqiong Li; Kunjie Li; Yulin Deng

doi:10.1007/s00604-018-2905-4

Aptamer-based fluorometric lateral flow assay for creatine kinase MB

Jing Zhang, Xuefei Lv, Wei Feng, Xiaoqiong Li^*, Kunjie Li, Yulin Deng

^*Corresponding author for this work

School of Medical and Technology

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

A group of aptamers possessing high specificity and affinity for creatine kinase MB (CKMB) was obtained by magnetic systematic evolution of ligands by exponential enrichment. Two aptamers (referred to as C.Apt.21 and C.Apt.30) were found to possess adequately low K_d values. They form a well suited pair for CKMB binding. By using fluorescent microspheres, an aptamer-based lateral flow assay was developed. It is portable, economical, and sensitive. The limit of detection for CKMB is as low as 0.63 ng·mL^-1, and the assay works in the 0.005 - 2 μg·mL^-1 CKMB concentration range. The method is specific for CKMB, and biomarkers for AMI (such as cardiac troponin I and myoglobin) and serum do not interfere. The strip is highly accurate as shown by analysis of spiked serum samples which gave recoveries ranging between 88 and 117%.

Original language	English
Article number	364
Journal	Microchimica Acta
Volume	185
Issue number	8
DOIs	https://doi.org/10.1007/s00604-018-2905-4
Publication status	Published - 1 Aug 2018

Keywords

Acute myocardial infarction
Aptamer
CKMB
Fluorescent microsphere
Lateral flow
Magnetic SELEX
Nanoparticles
Rapid detection
Sandwich
Test strip reader

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title = "Aptamer-based fluorometric lateral flow assay for creatine kinase MB",

abstract = "A group of aptamers possessing high specificity and affinity for creatine kinase MB (CKMB) was obtained by magnetic systematic evolution of ligands by exponential enrichment. Two aptamers (referred to as C.Apt.21 and C.Apt.30) were found to possess adequately low Kd values. They form a well suited pair for CKMB binding. By using fluorescent microspheres, an aptamer-based lateral flow assay was developed. It is portable, economical, and sensitive. The limit of detection for CKMB is as low as 0.63 ng·mL-1, and the assay works in the 0.005 - 2 μg·mL-1 CKMB concentration range. The method is specific for CKMB, and biomarkers for AMI (such as cardiac troponin I and myoglobin) and serum do not interfere. The strip is highly accurate as shown by analysis of spiked serum samples which gave recoveries ranging between 88 and 117%.",

keywords = "Acute myocardial infarction, Aptamer, CKMB, Fluorescent microsphere, Lateral flow, Magnetic SELEX, Nanoparticles, Rapid detection, Sandwich, Test strip reader",

author = "Jing Zhang and Xuefei Lv and Wei Feng and Xiaoqiong Li and Kunjie Li and Yulin Deng",

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AU - Zhang, Jing

AU - Lv, Xuefei

AU - Feng, Wei

AU - Li, Xiaoqiong

AU - Li, Kunjie

AU - Deng, Yulin

PY - 2018/8/1

Y1 - 2018/8/1

N2 - A group of aptamers possessing high specificity and affinity for creatine kinase MB (CKMB) was obtained by magnetic systematic evolution of ligands by exponential enrichment. Two aptamers (referred to as C.Apt.21 and C.Apt.30) were found to possess adequately low Kd values. They form a well suited pair for CKMB binding. By using fluorescent microspheres, an aptamer-based lateral flow assay was developed. It is portable, economical, and sensitive. The limit of detection for CKMB is as low as 0.63 ng·mL-1, and the assay works in the 0.005 - 2 μg·mL-1 CKMB concentration range. The method is specific for CKMB, and biomarkers for AMI (such as cardiac troponin I and myoglobin) and serum do not interfere. The strip is highly accurate as shown by analysis of spiked serum samples which gave recoveries ranging between 88 and 117%.

AB - A group of aptamers possessing high specificity and affinity for creatine kinase MB (CKMB) was obtained by magnetic systematic evolution of ligands by exponential enrichment. Two aptamers (referred to as C.Apt.21 and C.Apt.30) were found to possess adequately low Kd values. They form a well suited pair for CKMB binding. By using fluorescent microspheres, an aptamer-based lateral flow assay was developed. It is portable, economical, and sensitive. The limit of detection for CKMB is as low as 0.63 ng·mL-1, and the assay works in the 0.005 - 2 μg·mL-1 CKMB concentration range. The method is specific for CKMB, and biomarkers for AMI (such as cardiac troponin I and myoglobin) and serum do not interfere. The strip is highly accurate as shown by analysis of spiked serum samples which gave recoveries ranging between 88 and 117%.

KW - Acute myocardial infarction

KW - Aptamer

KW - CKMB

KW - Fluorescent microsphere

KW - Lateral flow

KW - Magnetic SELEX

KW - Nanoparticles

KW - Rapid detection

KW - Sandwich

KW - Test strip reader

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JO - Microchimica Acta

JF - Microchimica Acta

IS - 8

M1 - 364

ER -

Aptamer-based fluorometric lateral flow assay for creatine kinase MB

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Keywords

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