TY - JOUR
T1 - Application of an atmospheric pressure plasma jet in a rat model of ischaemic stroke
T2 - Design, optimisation, and characteristics
AU - Chen, Ye
AU - Yang, Bingyan
AU - Liu, Yuqing
AU - Xu, Lixin
AU - Shi, Zhongfang
AU - Liu, Yixiao
AU - Han, Ruoyu
AU - Yuan, Fang
AU - Ouyang, Jiting
AU - Yan, Xu
AU - Ostrikov, Kostya
N1 - Publisher Copyright:
© 2022 The Authors. High Voltage published by John Wiley & Sons Ltd on behalf of The Institution of Engineering and Technology and China Electric Power Research Institute.
PY - 2023/4
Y1 - 2023/4
N2 - Recent studies have shown the in vitro neuro-protective functions of atmospheric pressure plasma (APP) against multiple pathological injuries during ischaemic stroke (IS). However, whether APP treatment exerts a therapeutic effect on a rat IS model remains unclear. Here, on the basis of needle-to-ring dielectric barrier discharge, an atmospheric pressure plasma jet (APPJ) was designed, with the Helium as the working gas which was driven by a sinusoidal voltage. Then, the treatment conditions were optimised for IS rat model treatment and the characteristics of this APPJ were further diagnosed. Subsequently, the rat IS model was established through 90 min middle cerebral artery occlusion (MCAO), and plasma was intermittently inhaled by rats via the nasal cavity for a 2 min period at 60 min of MCAO process. The therapeutic effects of this plasma jet device were then evaluated using biomedical analyses. According to our results, intermittent APP inhalation in the MCAO rats increased the serum NO content, improved the neurological function, enhanced regional cerebral blood flow, lowered brain infarction, and reduced the cell apoptosis in brain tissues of MCAO rats. Collectively, our data provides a novel potential strategy for IS treatment by using atmospheric-pressure plasma inhalation.
AB - Recent studies have shown the in vitro neuro-protective functions of atmospheric pressure plasma (APP) against multiple pathological injuries during ischaemic stroke (IS). However, whether APP treatment exerts a therapeutic effect on a rat IS model remains unclear. Here, on the basis of needle-to-ring dielectric barrier discharge, an atmospheric pressure plasma jet (APPJ) was designed, with the Helium as the working gas which was driven by a sinusoidal voltage. Then, the treatment conditions were optimised for IS rat model treatment and the characteristics of this APPJ were further diagnosed. Subsequently, the rat IS model was established through 90 min middle cerebral artery occlusion (MCAO), and plasma was intermittently inhaled by rats via the nasal cavity for a 2 min period at 60 min of MCAO process. The therapeutic effects of this plasma jet device were then evaluated using biomedical analyses. According to our results, intermittent APP inhalation in the MCAO rats increased the serum NO content, improved the neurological function, enhanced regional cerebral blood flow, lowered brain infarction, and reduced the cell apoptosis in brain tissues of MCAO rats. Collectively, our data provides a novel potential strategy for IS treatment by using atmospheric-pressure plasma inhalation.
UR - http://www.scopus.com/inward/record.url?scp=85140260562&partnerID=8YFLogxK
U2 - 10.1049/hve2.12267
DO - 10.1049/hve2.12267
M3 - Article
AN - SCOPUS:85140260562
SN - 2397-7264
VL - 8
SP - 315
EP - 325
JO - High Voltage
JF - High Voltage
IS - 2
ER -