TY - JOUR
T1 - Analysis of gingerol-related compounds in fresh ginger by HPLC-ESI-Q-TOF-MS/MS
AU - Wang, Li
AU - Fang, Lei
AU - Zhao, Hengqiang
AU - Wang, Shanshan
AU - Du, Jinhua
AU - Wang, Xiao
PY - 2011/12/15
Y1 - 2011/12/15
N2 - To establish a rapid method for analysis of gingerol-related compounds in fresh ginger by using high performance liquid chromatography coupled with electron spray ionization-quadrupole-time of flight mass/mass spectrometry (HPLC-ESI-Q-TOF-MS/MS). The gingerol-related compounds in fresh ginger was separated by an Inertsil ODS-SP column (4.6 mm x 250 mm, 5 μm) using a binary eluent under gradient conditions. The analytes were detected by ESI-Q-TOF-MS/MS in positive ion mode to obtain MS and MS/MS spectra and to extract molecular weights. From the MS data, the accurate molecular weights of gingerol-related compounds were obtained, and from the MS/MS data, the (+) ESI-Q-TOF-MS/MS fragments were obtained. 25 gingerol-related compounds were identified from fresh ginger by attentive studying on the mass spectra of compounds and comparing with reference data reported in the literature, respectively. This method was certified to be accurate and reliable and can be used for the rapid analysis of gingerol-related compounds in fresh ginger.
AB - To establish a rapid method for analysis of gingerol-related compounds in fresh ginger by using high performance liquid chromatography coupled with electron spray ionization-quadrupole-time of flight mass/mass spectrometry (HPLC-ESI-Q-TOF-MS/MS). The gingerol-related compounds in fresh ginger was separated by an Inertsil ODS-SP column (4.6 mm x 250 mm, 5 μm) using a binary eluent under gradient conditions. The analytes were detected by ESI-Q-TOF-MS/MS in positive ion mode to obtain MS and MS/MS spectra and to extract molecular weights. From the MS data, the accurate molecular weights of gingerol-related compounds were obtained, and from the MS/MS data, the (+) ESI-Q-TOF-MS/MS fragments were obtained. 25 gingerol-related compounds were identified from fresh ginger by attentive studying on the mass spectra of compounds and comparing with reference data reported in the literature, respectively. This method was certified to be accurate and reliable and can be used for the rapid analysis of gingerol-related compounds in fresh ginger.
KW - Fresh ginger
KW - Gingerol-related compounds
KW - HPLC-ESI-Q-TOF-MS/MS
UR - http://www.scopus.com/inward/record.url?scp=84863275656&partnerID=8YFLogxK
U2 - 10.4268/cjcmm20112417
DO - 10.4268/cjcmm20112417
M3 - Article
C2 - 22368858
AN - SCOPUS:84863275656
SN - 1001-5302
VL - 36
SP - 3467
EP - 3471
JO - Zhongguo Zhongyao Zazhi
JF - Zhongguo Zhongyao Zazhi
IS - 24
ER -