Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier

Shutao Guo; Yuanyu Huang; Tuo Wei; Wendi Zhang; Weiwei Wang; Daoshu Lin; Xu Zhang; Anil Kumar; Quan Du; Jinfeng Xing; Liandong Deng; Zicai Liang; Paul C. Wang; Anjie Dong; Xing Jie Liang

doi:10.1016/j.biomaterials.2010.09.052

Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier

Shutao Guo, Yuanyu Huang, Tuo Wei, Wendi Zhang, Weiwei Wang, Daoshu Lin, Xu Zhang, Anil Kumar, Quan Du, Jinfeng Xing, Liandong Deng, Zicai Liang, Paul C. Wang, Anjie Dong, Xing Jie Liang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

98 Citations (Scopus)

Abstract

A group of amphiphilic cationic polymers, methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) (PECD), were synthesized by combining ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP) methods to form nanoparticles (NPs). The structures of these amphiphilic cationic polymers were characterized by ¹H NMR measurement. The PECD NPs have hydrophobic cores covered with hydrophilic PEG and cationic PDMAEMA chains. These self-assembly nanoparticles were characterized by dynamic light scattering (DLS) technique. PECD NPs can effectively condense DNA to form compact complexes of the size 65-160 nm suitable for gene delivery. The in vitro gene transfection studies of HeLa and HepG2 cells show that PECD NPs have better transfection efficiency compared to polyethylenimine (PEI) and Lipofectamine 2000 at low dose (N/P = 5). The cytotoxicity result shows that PECD NPs/DNA complexes at the optimal N/P ratio for transfection have comparable toxicity with PEI and Lipofectamine. These results indicate that PECD NPs have a great potential to be used as efficient polymeric carriers for gene transfection.

Original language	English
Pages (from-to)	879-889
Number of pages	11
Journal	Biomaterials
Volume	32
Issue number	3
DOIs	https://doi.org/10.1016/j.biomaterials.2010.09.052
Publication status	Published - Jan 2011
Externally published	Yes

Keywords

Amphiphilic copolymers
Degradable polymers
Gene therapy
Poly(2-(dimethylamino ethyl)methacrylate)
Poly(e{open}-caprolactone)

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10.1016/j.biomaterials.2010.09.052

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Guo, S., Huang, Y., Wei, T., Zhang, W., Wang, W., Lin, D., Zhang, X., Kumar, A., Du, Q., Xing, J., Deng, L., Liang, Z., Wang, P. C., Dong, A., & Liang, X. J. (2011). Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier. Biomaterials, 32(3), 879-889. https://doi.org/10.1016/j.biomaterials.2010.09.052

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title = "Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier",

abstract = "A group of amphiphilic cationic polymers, methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) (PECD), were synthesized by combining ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP) methods to form nanoparticles (NPs). The structures of these amphiphilic cationic polymers were characterized by 1H NMR measurement. The PECD NPs have hydrophobic cores covered with hydrophilic PEG and cationic PDMAEMA chains. These self-assembly nanoparticles were characterized by dynamic light scattering (DLS) technique. PECD NPs can effectively condense DNA to form compact complexes of the size 65-160 nm suitable for gene delivery. The in vitro gene transfection studies of HeLa and HepG2 cells show that PECD NPs have better transfection efficiency compared to polyethylenimine (PEI) and Lipofectamine 2000 at low dose (N/P = 5). The cytotoxicity result shows that PECD NPs/DNA complexes at the optimal N/P ratio for transfection have comparable toxicity with PEI and Lipofectamine. These results indicate that PECD NPs have a great potential to be used as efficient polymeric carriers for gene transfection.",

keywords = "Amphiphilic copolymers, Degradable polymers, Gene therapy, Poly(2-(dimethylamino ethyl)methacrylate), Poly(e{open}-caprolactone)",

author = "Shutao Guo and Yuanyu Huang and Tuo Wei and Wendi Zhang and Weiwei Wang and Daoshu Lin and Xu Zhang and Anil Kumar and Quan Du and Jinfeng Xing and Liandong Deng and Zicai Liang and Wang, {Paul C.} and Anjie Dong and Liang, {Xing Jie}",

year = "2011",

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doi = "10.1016/j.biomaterials.2010.09.052",

language = "English",

volume = "32",

pages = "879--889",

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Guo, S, Huang, Y, Wei, T, Zhang, W, Wang, W, Lin, D, Zhang, X, Kumar, A, Du, Q, Xing, J, Deng, L, Liang, Z, Wang, PC, Dong, A & Liang, XJ 2011, 'Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier', Biomaterials, vol. 32, no. 3, pp. 879-889. https://doi.org/10.1016/j.biomaterials.2010.09.052

TY - JOUR

T1 - Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier

AU - Guo, Shutao

AU - Huang, Yuanyu

AU - Wei, Tuo

AU - Zhang, Wendi

AU - Wang, Weiwei

AU - Lin, Daoshu

AU - Zhang, Xu

AU - Kumar, Anil

AU - Du, Quan

AU - Xing, Jinfeng

AU - Deng, Liandong

AU - Liang, Zicai

AU - Wang, Paul C.

AU - Dong, Anjie

AU - Liang, Xing Jie

PY - 2011/1

Y1 - 2011/1

N2 - A group of amphiphilic cationic polymers, methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) (PECD), were synthesized by combining ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP) methods to form nanoparticles (NPs). The structures of these amphiphilic cationic polymers were characterized by 1H NMR measurement. The PECD NPs have hydrophobic cores covered with hydrophilic PEG and cationic PDMAEMA chains. These self-assembly nanoparticles were characterized by dynamic light scattering (DLS) technique. PECD NPs can effectively condense DNA to form compact complexes of the size 65-160 nm suitable for gene delivery. The in vitro gene transfection studies of HeLa and HepG2 cells show that PECD NPs have better transfection efficiency compared to polyethylenimine (PEI) and Lipofectamine 2000 at low dose (N/P = 5). The cytotoxicity result shows that PECD NPs/DNA complexes at the optimal N/P ratio for transfection have comparable toxicity with PEI and Lipofectamine. These results indicate that PECD NPs have a great potential to be used as efficient polymeric carriers for gene transfection.

AB - A group of amphiphilic cationic polymers, methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) (PECD), were synthesized by combining ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP) methods to form nanoparticles (NPs). The structures of these amphiphilic cationic polymers were characterized by 1H NMR measurement. The PECD NPs have hydrophobic cores covered with hydrophilic PEG and cationic PDMAEMA chains. These self-assembly nanoparticles were characterized by dynamic light scattering (DLS) technique. PECD NPs can effectively condense DNA to form compact complexes of the size 65-160 nm suitable for gene delivery. The in vitro gene transfection studies of HeLa and HepG2 cells show that PECD NPs have better transfection efficiency compared to polyethylenimine (PEI) and Lipofectamine 2000 at low dose (N/P = 5). The cytotoxicity result shows that PECD NPs/DNA complexes at the optimal N/P ratio for transfection have comparable toxicity with PEI and Lipofectamine. These results indicate that PECD NPs have a great potential to be used as efficient polymeric carriers for gene transfection.

KW - Amphiphilic copolymers

KW - Degradable polymers

KW - Gene therapy

KW - Poly(2-(dimethylamino ethyl)methacrylate)

KW - Poly(e{open}-caprolactone)

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U2 - 10.1016/j.biomaterials.2010.09.052

DO - 10.1016/j.biomaterials.2010.09.052

M3 - Article

C2 - 20970186

AN - SCOPUS:78549274320

SN - 0142-9612

VL - 32

SP - 879

EP - 889

JO - Biomaterials

JF - Biomaterials

IS - 3

ER -

Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier

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Keywords

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