Guo, S., Huang, Y., Wei, T., Zhang, W., Wang, W., Lin, D., Zhang, X., Kumar, A., Du, Q., Xing, J., Deng, L., Liang, Z., Wang, P. C., Dong, A., & Liang, X. J. (2011). Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier. Biomaterials, 32(3), 879-889. https://doi.org/10.1016/j.biomaterials.2010.09.052
Guo, Shutao ; Huang, Yuanyu ; Wei, Tuo et al. / Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier. In: Biomaterials. 2011 ; Vol. 32, No. 3. pp. 879-889.
@article{d8bc5475f79e4b8da05e892f8e14d802,
title = "Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier",
abstract = "A group of amphiphilic cationic polymers, methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) (PECD), were synthesized by combining ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP) methods to form nanoparticles (NPs). The structures of these amphiphilic cationic polymers were characterized by 1H NMR measurement. The PECD NPs have hydrophobic cores covered with hydrophilic PEG and cationic PDMAEMA chains. These self-assembly nanoparticles were characterized by dynamic light scattering (DLS) technique. PECD NPs can effectively condense DNA to form compact complexes of the size 65-160 nm suitable for gene delivery. The in vitro gene transfection studies of HeLa and HepG2 cells show that PECD NPs have better transfection efficiency compared to polyethylenimine (PEI) and Lipofectamine 2000 at low dose (N/P = 5). The cytotoxicity result shows that PECD NPs/DNA complexes at the optimal N/P ratio for transfection have comparable toxicity with PEI and Lipofectamine. These results indicate that PECD NPs have a great potential to be used as efficient polymeric carriers for gene transfection.",
keywords = "Amphiphilic copolymers, Degradable polymers, Gene therapy, Poly(2-(dimethylamino ethyl)methacrylate), Poly(e{open}-caprolactone)",
author = "Shutao Guo and Yuanyu Huang and Tuo Wei and Wendi Zhang and Weiwei Wang and Daoshu Lin and Xu Zhang and Anil Kumar and Quan Du and Jinfeng Xing and Liandong Deng and Zicai Liang and Wang, {Paul C.} and Anjie Dong and Liang, {Xing Jie}",
year = "2011",
month = jan,
doi = "10.1016/j.biomaterials.2010.09.052",
language = "English",
volume = "32",
pages = "879--889",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier Ltd.",
number = "3",
}
Guo, S, Huang, Y, Wei, T, Zhang, W, Wang, W, Lin, D, Zhang, X, Kumar, A, Du, Q, Xing, J, Deng, L, Liang, Z, Wang, PC, Dong, A & Liang, XJ 2011, 'Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier', Biomaterials, vol. 32, no. 3, pp. 879-889. https://doi.org/10.1016/j.biomaterials.2010.09.052
Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier. / Guo, Shutao
; Huang, Yuanyu; Wei, Tuo et al.
In:
Biomaterials, Vol. 32, No. 3, 01.2011, p. 879-889.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier
AU - Guo, Shutao
AU - Huang, Yuanyu
AU - Wei, Tuo
AU - Zhang, Wendi
AU - Wang, Weiwei
AU - Lin, Daoshu
AU - Zhang, Xu
AU - Kumar, Anil
AU - Du, Quan
AU - Xing, Jinfeng
AU - Deng, Liandong
AU - Liang, Zicai
AU - Wang, Paul C.
AU - Dong, Anjie
AU - Liang, Xing Jie
PY - 2011/1
Y1 - 2011/1
N2 - A group of amphiphilic cationic polymers, methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) (PECD), were synthesized by combining ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP) methods to form nanoparticles (NPs). The structures of these amphiphilic cationic polymers were characterized by 1H NMR measurement. The PECD NPs have hydrophobic cores covered with hydrophilic PEG and cationic PDMAEMA chains. These self-assembly nanoparticles were characterized by dynamic light scattering (DLS) technique. PECD NPs can effectively condense DNA to form compact complexes of the size 65-160 nm suitable for gene delivery. The in vitro gene transfection studies of HeLa and HepG2 cells show that PECD NPs have better transfection efficiency compared to polyethylenimine (PEI) and Lipofectamine 2000 at low dose (N/P = 5). The cytotoxicity result shows that PECD NPs/DNA complexes at the optimal N/P ratio for transfection have comparable toxicity with PEI and Lipofectamine. These results indicate that PECD NPs have a great potential to be used as efficient polymeric carriers for gene transfection.
AB - A group of amphiphilic cationic polymers, methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) (PECD), were synthesized by combining ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP) methods to form nanoparticles (NPs). The structures of these amphiphilic cationic polymers were characterized by 1H NMR measurement. The PECD NPs have hydrophobic cores covered with hydrophilic PEG and cationic PDMAEMA chains. These self-assembly nanoparticles were characterized by dynamic light scattering (DLS) technique. PECD NPs can effectively condense DNA to form compact complexes of the size 65-160 nm suitable for gene delivery. The in vitro gene transfection studies of HeLa and HepG2 cells show that PECD NPs have better transfection efficiency compared to polyethylenimine (PEI) and Lipofectamine 2000 at low dose (N/P = 5). The cytotoxicity result shows that PECD NPs/DNA complexes at the optimal N/P ratio for transfection have comparable toxicity with PEI and Lipofectamine. These results indicate that PECD NPs have a great potential to be used as efficient polymeric carriers for gene transfection.
KW - Amphiphilic copolymers
KW - Degradable polymers
KW - Gene therapy
KW - Poly(2-(dimethylamino ethyl)methacrylate)
KW - Poly(e{open}-caprolactone)
UR - http://www.scopus.com/inward/record.url?scp=78549274320&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2010.09.052
DO - 10.1016/j.biomaterials.2010.09.052
M3 - Article
C2 - 20970186
AN - SCOPUS:78549274320
SN - 0142-9612
VL - 32
SP - 879
EP - 889
JO - Biomaterials
JF - Biomaterials
IS - 3
ER -
Guo S, Huang Y, Wei T, Zhang W, Wang W, Lin D et al. Amphiphilic and biodegradable methoxy polyethylene glycol-block-(polycaprolactone-graft-poly(2-(dimethylamino)ethyl methacrylate)) as an effective gene carrier. Biomaterials. 2011 Jan;32(3):879-889. doi: 10.1016/j.biomaterials.2010.09.052
