Alterations of fetal brain biogenic amine metabolites and monoamine oxidase activity after ethanol treatment

Jian Mao; Yang Su; Xuechai Chen; Kaifei Dou; Yulin Deng

Alterations of fetal brain biogenic amine metabolites and monoamine oxidase activity after ethanol treatment

Jian Mao^*, Yang Su, Xuechai Chen, Kaifei Dou, Yulin Deng

^*Corresponding author for this work

School of Medical and Technology

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Pregnant SD rats were exposed to ethanol to assess whether ethanol treatment could alter monoamine transmitter metabolites and the activity of monoamine oxidase (MAO) in fetal rat brain. The activity of MAO was determined by HPLC-MS with time of flight detector,and the whole brain levels of dopamine (DA), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) were determined by HPLC with electrochemical detector. Ethanol administration inhibited the activity of mitochondrial MAO in fetal brain. The levels of DA and 5-HT in whole fetal brain were not remarkably altered by ethanol treatment, while the levels of HVA and 5HIAA in high dose (4.0g/kg) of ethanol-treated rats were significantly decreased compared to that in the control animals. The reduction of MAO activity and the alteration of DA and 5-HT metabolites may play a role in the central nervous system defects of alcohol teratogenicity.

Original language	English
Pages (from-to)	761-764
Number of pages	4
Journal	Chemistry Bulletin / Huaxue Tongbao
Volume	73
Issue number	8
Publication status	Published - 2010

Keywords

5-Hydroxytryptamine
Dopamine
HPLC
Monoamine oxidase

Cite this

Mao, J., Su, Y., Chen, X., Dou, K., & Deng, Y. (2010). Alterations of fetal brain biogenic amine metabolites and monoamine oxidase activity after ethanol treatment. Chemistry Bulletin / Huaxue Tongbao, 73(8), 761-764.

@article{434b3f190b284c6ca6acbcd929eab0af,

title = "Alterations of fetal brain biogenic amine metabolites and monoamine oxidase activity after ethanol treatment",

abstract = "Pregnant SD rats were exposed to ethanol to assess whether ethanol treatment could alter monoamine transmitter metabolites and the activity of monoamine oxidase (MAO) in fetal rat brain. The activity of MAO was determined by HPLC-MS with time of flight detector,and the whole brain levels of dopamine (DA), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) were determined by HPLC with electrochemical detector. Ethanol administration inhibited the activity of mitochondrial MAO in fetal brain. The levels of DA and 5-HT in whole fetal brain were not remarkably altered by ethanol treatment, while the levels of HVA and 5HIAA in high dose (4.0g/kg) of ethanol-treated rats were significantly decreased compared to that in the control animals. The reduction of MAO activity and the alteration of DA and 5-HT metabolites may play a role in the central nervous system defects of alcohol teratogenicity.",

keywords = "5-Hydroxytryptamine, Dopamine, HPLC, Monoamine oxidase",

author = "Jian Mao and Yang Su and Xuechai Chen and Kaifei Dou and Yulin Deng",

year = "2010",

language = "English",

volume = "73",

pages = "761--764",

journal = "Chemistry Bulletin / Huaxue Tongbao",

issn = "0441-3776",

publisher = "Editorial Office of Huaxue Tongbao",

number = "8",

}

TY - JOUR

T1 - Alterations of fetal brain biogenic amine metabolites and monoamine oxidase activity after ethanol treatment

AU - Mao, Jian

AU - Su, Yang

AU - Chen, Xuechai

AU - Dou, Kaifei

AU - Deng, Yulin

PY - 2010

Y1 - 2010

N2 - Pregnant SD rats were exposed to ethanol to assess whether ethanol treatment could alter monoamine transmitter metabolites and the activity of monoamine oxidase (MAO) in fetal rat brain. The activity of MAO was determined by HPLC-MS with time of flight detector,and the whole brain levels of dopamine (DA), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) were determined by HPLC with electrochemical detector. Ethanol administration inhibited the activity of mitochondrial MAO in fetal brain. The levels of DA and 5-HT in whole fetal brain were not remarkably altered by ethanol treatment, while the levels of HVA and 5HIAA in high dose (4.0g/kg) of ethanol-treated rats were significantly decreased compared to that in the control animals. The reduction of MAO activity and the alteration of DA and 5-HT metabolites may play a role in the central nervous system defects of alcohol teratogenicity.

AB - Pregnant SD rats were exposed to ethanol to assess whether ethanol treatment could alter monoamine transmitter metabolites and the activity of monoamine oxidase (MAO) in fetal rat brain. The activity of MAO was determined by HPLC-MS with time of flight detector,and the whole brain levels of dopamine (DA), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) were determined by HPLC with electrochemical detector. Ethanol administration inhibited the activity of mitochondrial MAO in fetal brain. The levels of DA and 5-HT in whole fetal brain were not remarkably altered by ethanol treatment, while the levels of HVA and 5HIAA in high dose (4.0g/kg) of ethanol-treated rats were significantly decreased compared to that in the control animals. The reduction of MAO activity and the alteration of DA and 5-HT metabolites may play a role in the central nervous system defects of alcohol teratogenicity.

KW - 5-Hydroxytryptamine

KW - Dopamine

KW - HPLC

KW - Monoamine oxidase

UR - http://www.scopus.com/inward/record.url?scp=78549274756&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:78549274756

SN - 0441-3776

VL - 73

SP - 761

EP - 764

JO - Chemistry Bulletin / Huaxue Tongbao

JF - Chemistry Bulletin / Huaxue Tongbao

IS - 8

ER -

Alterations of fetal brain biogenic amine metabolites and monoamine oxidase activity after ethanol treatment

Abstract

Keywords

Other files and links

Fingerprint

Cite this