Abstract
Microfluidic devices have shown capabilities for precise cell manipulation and analysis for decades. In recent years, the improvement of microfabrication technology and the introduction of new materials have boosted innovation on single-cell electroporation on microfluidic devices. This has opened the door for gene transfection and drug delivery with high efficiency and cellular safety in the exploration of cell biology and life science. In this chapter, we introduce the basic mechanism and state of the art of electroporation techniques based on microfluidic devices. We first discuss three types of electroporation systems for high-throughput cell transfection, including bulk electroporation, tubular microfluidic electroporation, and a localized electroporation device. Next, we shed light on cell manipulation techniques integrated on microfluidic electroporation devices to achieve higher efficiency and safer electroporation. Several methods for cell manipulation and their applications in electroporation for biomedicine are introduced.
| Original language | English |
|---|---|
| Title of host publication | Micro and Nano Systems for Biophysical Studies of Cells and Small Organisms |
| Publisher | Elsevier |
| Pages | 105-123 |
| Number of pages | 19 |
| ISBN (Electronic) | 9780128239902 |
| DOIs | |
| Publication status | Published - 1 Jan 2021 |
Keywords
- Cell manipulation
- Dielectrophoresis
- Electroporation
- Hydrodynamics
- Lab-on-a-chip
- Magnetic tweezers
- Microfluidic device
- Nanoelectroporation
- Optical tweezers