A study of cross-linkage of formaldehyde with human serum albumin

Bo Li, Lili Li, Dingyu Hu, Bo Peng, Qin Li, Shengyuan Xiao, Yujuan Li, Yulin Deng*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

It's well known that irreversible cross-linked complexes between proteins, produced by formaldehyde interacting with free amino or amide groups to form Schiff's base, are formed in diabetes and aging patients. To identify such specific modified peptides as biomarkers for clinical diagnose of diabetes and AD, cross-linkage of formaldehyde with Human Serum Albumin (HSA) was analyzed by LC/ESI/MS in this study. Eighteen peptides as important biomarkers were identified and the corresponding possible structures of their cross-linkages were also analyzed. Five of those peptides were modified by the reaction of one molecular proportion of formaldehyde with one molecular proportion of amino group. Reaction of one molecular proportion of formaldehyde with two molecular proportions of amino groups were found in 4 of the 18 peptides. In the rest 9 peptides the structures of the crosslinkages of formaldehyde with amino groups was more complex. Furthermore, several lysine and arginine residues of those peptides were proved as modification sites in HSA cross-linked with formaldehyde.

Original languageEnglish
Title of host publication3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
DOIs
Publication statusPublished - 2009
Event3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 - Beijing, China
Duration: 11 Jun 200913 Jun 2009

Publication series

Name3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009

Conference

Conference3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
Country/TerritoryChina
CityBeijing
Period11/06/0913/06/09

Keywords

  • Biomarker
  • Cross-linkage
  • LC/ESI/MS
  • Modification sites

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Li, B., Li, L., Hu, D., Peng, B., Li, Q., Xiao, S., Li, Y., & Deng, Y. (2009). A study of cross-linkage of formaldehyde with human serum albumin. In 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 Article 5162247 (3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009). https://doi.org/10.1109/ICBBE.2009.5162247