TY - GEN
T1 - A protein detection POCT device based on microfluidic immunoassay chips
AU - Yu, Lijia
AU - Li, Xiaoqiong
AU - Hu, Xiaoming
AU - Yu, Shiyong
AU - Lv, Xuefei
AU - Li, Qin
AU - Deng, Yulin
N1 - Publisher Copyright:
© 2015 IEEE.
PY - 2016/2/8
Y1 - 2016/2/8
N2 - Immunoassay is one of the most important protein detection methods, which is widely used in clinical diagnosis, environment monitor and biological research due to its high specificity and sensitivity. Most of the existing protein immunoassay detection devices have the weakness of high expenses, large scale and low sample processing rate. Microfluidic immunoassay chips have been widely recognized as a powerful technology that will play a vital role in future protein detection to meet low reagent, short reaction time and power consumption. In this paper, a protein detection point of care testing (POCT) device was presented aiming at the needs of detection with high-throughput, small size and site analysis. And a LED-induced scanning confocal fluorescence detection system was adopted in the device to achieve high sensitivity detection and small size. To facilitate system integration and data processing, a MSP430 microcontroller was used as the core chip. And a graphical user interface (GUI) software was provided to control the data acquiring, storing, processing and image display. Ultimately, a fluorescence image was acquired at a concentration of 1×10-8mol/L fluorescein isothiocyanate (FITC) solution by the device.
AB - Immunoassay is one of the most important protein detection methods, which is widely used in clinical diagnosis, environment monitor and biological research due to its high specificity and sensitivity. Most of the existing protein immunoassay detection devices have the weakness of high expenses, large scale and low sample processing rate. Microfluidic immunoassay chips have been widely recognized as a powerful technology that will play a vital role in future protein detection to meet low reagent, short reaction time and power consumption. In this paper, a protein detection point of care testing (POCT) device was presented aiming at the needs of detection with high-throughput, small size and site analysis. And a LED-induced scanning confocal fluorescence detection system was adopted in the device to achieve high sensitivity detection and small size. To facilitate system integration and data processing, a MSP430 microcontroller was used as the core chip. And a graphical user interface (GUI) software was provided to control the data acquiring, storing, processing and image display. Ultimately, a fluorescence image was acquired at a concentration of 1×10-8mol/L fluorescein isothiocyanate (FITC) solution by the device.
KW - confocalfluorescence detection
KW - microfluidic immunoassay chips
KW - protein detection device
UR - http://www.scopus.com/inward/record.url?scp=84964334137&partnerID=8YFLogxK
U2 - 10.1109/BMEI.2015.7401524
DO - 10.1109/BMEI.2015.7401524
M3 - Conference contribution
AN - SCOPUS:84964334137
T3 - Proceedings - 2015 8th International Conference on BioMedical Engineering and Informatics, BMEI 2015
SP - 328
EP - 332
BT - Proceedings - 2015 8th International Conference on BioMedical Engineering and Informatics, BMEI 2015
A2 - Tao, Zhiyong
A2 - Bai, Li
A2 - Lin, Sen
A2 - Sun, Jinguang
A2 - Wang, Lipo
A2 - Shao, Liangshan
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 8th International Conference on BioMedical Engineering and Informatics, BMEI 2015
Y2 - 14 October 2015 through 16 October 2015
ER -