A protein detection POCT device based on microfluidic immunoassay chips

Lijia Yu, Xiaoqiong Li, Xiaoming Hu, Shiyong Yu, Xuefei Lv, Qin Li*, Yulin Deng

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Immunoassay is one of the most important protein detection methods, which is widely used in clinical diagnosis, environment monitor and biological research due to its high specificity and sensitivity. Most of the existing protein immunoassay detection devices have the weakness of high expenses, large scale and low sample processing rate. Microfluidic immunoassay chips have been widely recognized as a powerful technology that will play a vital role in future protein detection to meet low reagent, short reaction time and power consumption. In this paper, a protein detection point of care testing (POCT) device was presented aiming at the needs of detection with high-throughput, small size and site analysis. And a LED-induced scanning confocal fluorescence detection system was adopted in the device to achieve high sensitivity detection and small size. To facilitate system integration and data processing, a MSP430 microcontroller was used as the core chip. And a graphical user interface (GUI) software was provided to control the data acquiring, storing, processing and image display. Ultimately, a fluorescence image was acquired at a concentration of 1×10-8mol/L fluorescein isothiocyanate (FITC) solution by the device.

Original languageEnglish
Title of host publicationProceedings - 2015 8th International Conference on BioMedical Engineering and Informatics, BMEI 2015
EditorsZhiyong Tao, Li Bai, Sen Lin, Jinguang Sun, Lipo Wang, Liangshan Shao
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages328-332
Number of pages5
ISBN (Electronic)9781509000227
DOIs
Publication statusPublished - 8 Feb 2016
Event8th International Conference on BioMedical Engineering and Informatics, BMEI 2015 - Shenyang, China
Duration: 14 Oct 201516 Oct 2015

Publication series

NameProceedings - 2015 8th International Conference on BioMedical Engineering and Informatics, BMEI 2015

Conference

Conference8th International Conference on BioMedical Engineering and Informatics, BMEI 2015
Country/TerritoryChina
CityShenyang
Period14/10/1516/10/15

Keywords

  • confocalfluorescence detection
  • microfluidic immunoassay chips
  • protein detection device

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Yu, L., Li, X., Hu, X., Yu, S., Lv, X., Li, Q., & Deng, Y. (2016). A protein detection POCT device based on microfluidic immunoassay chips. In Z. Tao, L. Bai, S. Lin, J. Sun, L. Wang, & L. Shao (Eds.), Proceedings - 2015 8th International Conference on BioMedical Engineering and Informatics, BMEI 2015 (pp. 328-332). Article 7401524 (Proceedings - 2015 8th International Conference on BioMedical Engineering and Informatics, BMEI 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/BMEI.2015.7401524