TY - GEN
T1 - A portable and high efficiency system for cell electroporation under low voltage
AU - Wu, Mengxi
AU - Wei, Zewen
AU - Zhao, Deyao
AU - Li, Zhihong
PY - 2011
Y1 - 2011
N2 - Based on a novel micro-fabricated electroporation (EP) chip, a portable EP system was designed and manufactured. The whole system consisted of a programmable and adjustable mini impulsator and micro EP chips. Large statistical data of transfection efficiency were determined under various pulse amplitudes, durations and intervals in this system for HEK-293 cells. High transfeciton efficiency (80%) was achieved under low voltage (∼20V) in this system. A man-machine interface was developed by keyboard and LCD screen in the mini impulsator using single chip micyoco. The impulsator can be adjusted to accommodate its pulse electrical parameters to a variety of conditions. The system has been fabricated and is in probation. With further EP experiments on other kinds of cells, more guidance will be given for researchers to shorten EP time and enhance efficiency.
AB - Based on a novel micro-fabricated electroporation (EP) chip, a portable EP system was designed and manufactured. The whole system consisted of a programmable and adjustable mini impulsator and micro EP chips. Large statistical data of transfection efficiency were determined under various pulse amplitudes, durations and intervals in this system for HEK-293 cells. High transfeciton efficiency (80%) was achieved under low voltage (∼20V) in this system. A man-machine interface was developed by keyboard and LCD screen in the mini impulsator using single chip micyoco. The impulsator can be adjusted to accommodate its pulse electrical parameters to a variety of conditions. The system has been fabricated and is in probation. With further EP experiments on other kinds of cells, more guidance will be given for researchers to shorten EP time and enhance efficiency.
KW - HEK-293 cell
KW - electroporation
KW - low voltage transfer
KW - man-machine interface
KW - single chip micyoco system
UR - http://www.scopus.com/inward/record.url?scp=80053324924&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2011.6017467
DO - 10.1109/NEMS.2011.6017467
M3 - Conference contribution
AN - SCOPUS:80053324924
SN - 9781612847757
T3 - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
SP - 768
EP - 771
BT - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
T2 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Y2 - 20 February 2011 through 23 February 2011
ER -
