A novel method to enhance sensitivity in HPLC detection

Yuxia Wei, Shengyuan Xiao, Xiaoling Mu, Yong Zhu, Gaofei Hu, Yulin Deng*, Xinlin Yang

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The peak compression effect induced by velocity difference has been theoretically and experimentally established. L-tryptophan and 2-nitrophenol were used as model compounds. The peak compression of L-tryptophan and 2-nitrophenol was obtained by the difference of its migration velocity along the column induced by the adjustment of composition of mobile phase. A compression of analytes peak and reduced tailing was observed. Under optimum compression condition, 17-fold and 5.9-fold improvement in the detection sensitivity was obtained compared to elution under non-compressed conditions for L-tryptophan and 2-nitrophenol, respectively.

Original languageEnglish
Title of host publication2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Pages1770-1774
Number of pages5
DOIs
Publication statusPublished - 2007
Event2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 - Beijing, China
Duration: 23 May 200727 May 2007

Publication series

Name2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

Conference

Conference2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Country/TerritoryChina
CityBeijing
Period23/05/0727/05/07

Keywords

  • Detection
  • HPLC
  • Sensitivity

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