A Microfluidic Chip for Screening High-Producing Hybridomas at the Single-Cell Level

Wei K. Zhang; Ren Li; Zhi G. Fang; Zhi Y. Hu; Ze W. Wei; Qin Li

doi:10.1109/MEMSYS.2019.8870728

A Microfluidic Chip for Screening High-Producing Hybridomas at the Single-Cell Level

Wei K. Zhang, Ren Li, Zhi G. Fang, Zhi Y. Hu, Ze W. Wei, Qin Li

School of Medical and Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Screening high-producing antibody-secreting cell from heterogeneity mammalian cell line populations is the vital procedure during the long time culture for antibody manufacture. To acquire high-producing cells, we designed a novel microfluidic chip for screening high-producing hybridomas from heterogeneous population at single cell level. All the procedures of hybridoma screening, including single cell trapping, identification, culturing and releasing, are integrated in a single chip. Compared with traditional methods based on limiting dilution, our chip realized higher monoclonal antibody (mAb) yield and shortened the screening period from 10 to 5 days with twice. It is significance that provides foundation to pharmaceutical company of rapidly screening cell lines.

Original language	English
Title of host publication	2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	422-425
Number of pages	4
ISBN (Electronic)	9781728116105
DOIs	https://doi.org/10.1109/MEMSYS.2019.8870728
Publication status	Published - Jan 2019
Event	32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019 - Seoul, Korea, Republic of Duration: 27 Jan 2019 → 31 Jan 2019

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2019-January
ISSN (Print)	1084-6999

Conference

Conference	32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019
Country/Territory	Korea, Republic of
City	Seoul
Period	27/01/19 → 31/01/19

Access to Document

10.1109/MEMSYS.2019.8870728

Cite this

Zhang, W. K., Li, R., Fang, Z. G., Hu, Z. Y., Wei, Z. W., & Li, Q. (2019). A Microfluidic Chip for Screening High-Producing Hybridomas at the Single-Cell Level. In 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019 (pp. 422-425). Article 8870728 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2019-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2019.8870728

Zhang, Wei K. ; Li, Ren ; Fang, Zhi G. et al. / A Microfluidic Chip for Screening High-Producing Hybridomas at the Single-Cell Level. 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 422-425 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

@inproceedings{812ca4382bd2424b84c4b9a520c65d70,

title = "A Microfluidic Chip for Screening High-Producing Hybridomas at the Single-Cell Level",

abstract = "Screening high-producing antibody-secreting cell from heterogeneity mammalian cell line populations is the vital procedure during the long time culture for antibody manufacture. To acquire high-producing cells, we designed a novel microfluidic chip for screening high-producing hybridomas from heterogeneous population at single cell level. All the procedures of hybridoma screening, including single cell trapping, identification, culturing and releasing, are integrated in a single chip. Compared with traditional methods based on limiting dilution, our chip realized higher monoclonal antibody (mAb) yield and shortened the screening period from 10 to 5 days with twice. It is significance that provides foundation to pharmaceutical company of rapidly screening cell lines.",

author = "Zhang, {Wei K.} and Ren Li and Fang, {Zhi G.} and Hu, {Zhi Y.} and Wei, {Ze W.} and Qin Li",

note = "Publisher Copyright: {\textcopyright} 2019 IEEE.; 32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019 ; Conference date: 27-01-2019 Through 31-01-2019",

year = "2019",

month = jan,

doi = "10.1109/MEMSYS.2019.8870728",

language = "English",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "422--425",

booktitle = "2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019",

address = "United States",

}

Zhang, WK, Li, R, Fang, ZG, Hu, ZY, Wei, ZW & Li, Q 2019, A Microfluidic Chip for Screening High-Producing Hybridomas at the Single-Cell Level. in 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019., 8870728, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2019-January, Institute of Electrical and Electronics Engineers Inc., pp. 422-425, 32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019, Seoul, Korea, Republic of, 27/01/19. https://doi.org/10.1109/MEMSYS.2019.8870728

A Microfluidic Chip for Screening High-Producing Hybridomas at the Single-Cell Level. / Zhang, Wei K.; Li, Ren; Fang, Zhi G. et al.
2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019. Institute of Electrical and Electronics Engineers Inc., 2019. p. 422-425 8870728 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2019-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AB - Screening high-producing antibody-secreting cell from heterogeneity mammalian cell line populations is the vital procedure during the long time culture for antibody manufacture. To acquire high-producing cells, we designed a novel microfluidic chip for screening high-producing hybridomas from heterogeneous population at single cell level. All the procedures of hybridoma screening, including single cell trapping, identification, culturing and releasing, are integrated in a single chip. Compared with traditional methods based on limiting dilution, our chip realized higher monoclonal antibody (mAb) yield and shortened the screening period from 10 to 5 days with twice. It is significance that provides foundation to pharmaceutical company of rapidly screening cell lines.

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Zhang WK, Li R, Fang ZG, Hu ZY, Wei ZW , Li Q. A Microfluidic Chip for Screening High-Producing Hybridomas at the Single-Cell Level. In 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019. Institute of Electrical and Electronics Engineers Inc. 2019. p. 422-425. 8870728. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2019.8870728

A Microfluidic Chip for Screening High-Producing Hybridomas at the Single-Cell Level

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