Abstract
Here we report a novel method to detect multiple SARS-CoV-2 variants in 70 min by a single microfluidic chip. By integrating virus lysis, nucleic acid extraction, amplification and CRISPR/Cas fluorescence detection in a three-channel microfluidic chip, the existence of different SARS-CoV-2 variants can be accurately detected and visually identified, avoiding the sequencing process.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 658-659 |
| Number of pages | 2 |
| ISBN (Electronic) | 9781733419048 |
| Publication status | Published - 2022 |
| Event | 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 - Hybrid, Hangzhou, China Duration: 23 Oct 2022 → 27 Oct 2022 |
Publication series
| Name | MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Conference
| Conference | 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 |
|---|---|
| Country/Territory | China |
| City | Hybrid, Hangzhou |
| Period | 23/10/22 → 27/10/22 |
Keywords
- CRISPR/CAS
- SARS-CoV-2 variants
- microfluidic
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Li, W., & Wei, Z. (2022). A MICROFLUIDIC CHIP FOR DETECTING MULTIPLE SARS-CoV-2 VARIANTS BASED ON CRISPR/CAS SYSTEM. In MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 658-659). (MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.