TY - GEN
T1 - 3D magnetic assembly of cellular structures with 'printing' manipulation by microrobot-controlled microfluidic system
AU - Li, Pengyun
AU - Shi, Qing
AU - Wang, Huaping
AU - Tu, Xiaolan
AU - Sun, Tao
AU - Liu, Xiaoming
AU - Huang, Qiang
AU - Fukuda, Toshio
N1 - Publisher Copyright:
© 2015 IEEE.
PY - 2015
Y1 - 2015
N2 - Microfluidic technology provides a mild way to fabricate cell-laden hydrogel three-dimensional (3D) modules. However, the poor controllability of hydrogels and unstable microfluidic fabrication process are still problems during the assembly process. In this paper, a novel 'bottom-up' method is used to assemble the complex shapes with magnetic alginate microflbers (MAMs) under an optimized magnetic field and a robotic assembly system. MAMs encapsulating Fe3O4 magnetic nanoparticles (MNs) and fibroblasts (NIH/3T3) are spun and automatically assembled under the magnetic field produced by a ring magnet. Experimental results show that the MAMs can respond quickly to the magnetic field, which enhances the controllability of hydrogels. The spinning process can be more stable with immersing the spinning orifice of microfluidic device into deionized water and using the 'pinch-off' scheme to wash the blockage off. Moreover, an optimization of magnetic assembly area of ring magnet is completed. Because of magnetic field, the complex assembly can be completed just by controlling robot arm which fixes the microfluidic device to move in XY plane. The secondary cross-linking method is employed to shape the structure on the support model. Finally, from the LIVE/DEAD assay, cells can survive well during the magnetic assembly process.
AB - Microfluidic technology provides a mild way to fabricate cell-laden hydrogel three-dimensional (3D) modules. However, the poor controllability of hydrogels and unstable microfluidic fabrication process are still problems during the assembly process. In this paper, a novel 'bottom-up' method is used to assemble the complex shapes with magnetic alginate microflbers (MAMs) under an optimized magnetic field and a robotic assembly system. MAMs encapsulating Fe3O4 magnetic nanoparticles (MNs) and fibroblasts (NIH/3T3) are spun and automatically assembled under the magnetic field produced by a ring magnet. Experimental results show that the MAMs can respond quickly to the magnetic field, which enhances the controllability of hydrogels. The spinning process can be more stable with immersing the spinning orifice of microfluidic device into deionized water and using the 'pinch-off' scheme to wash the blockage off. Moreover, an optimization of magnetic assembly area of ring magnet is completed. Because of magnetic field, the complex assembly can be completed just by controlling robot arm which fixes the microfluidic device to move in XY plane. The secondary cross-linking method is employed to shape the structure on the support model. Finally, from the LIVE/DEAD assay, cells can survive well during the magnetic assembly process.
UR - http://www.scopus.com/inward/record.url?scp=84964501422&partnerID=8YFLogxK
U2 - 10.1109/ROBIO.2015.7419061
DO - 10.1109/ROBIO.2015.7419061
M3 - Conference contribution
AN - SCOPUS:84964501422
T3 - 2015 IEEE International Conference on Robotics and Biomimetics, IEEE-ROBIO 2015
SP - 1967
EP - 1972
BT - 2015 IEEE International Conference on Robotics and Biomimetics, IEEE-ROBIO 2015
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - IEEE International Conference on Robotics and Biomimetics, IEEE-ROBIO 2015
Y2 - 6 December 2015 through 9 December 2015
ER -