乙酰胆碱酯酶纳米胶囊的合成与稳定性研究

Chengzhao Tu; Meiling Lei; Qingping Cheng; Lin Ye

doi:10.11730/j.issn.1001-7631.2020.04.0246.07

乙酰胆碱酯酶纳米胶囊的合成与稳定性研究

Translated title of the contribution: Synthesis and Stability of Acetylcholinesterase Nanocapsules

Chengzhao Tu, Meiling Lei^*, Qingping Cheng, Lin Ye

^*Corresponding author for this work

School of Material Science and Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Acetylcholinesterase nanocapsules (nAChE) were prepared by two-step method. Firstly, n-acryloyloxysuccinimide was used to introduce double bonds on the surface of AChE. Then, N-(3-aminopropyl) methacrylate and acrylamide were used as monomers, and N,N'-methylene bisacrylamide was used as crosslinking agent to prepare AChE nanocapsules. The results of dynamic light scattering (DLS) showed that the particle size of AChE nanocapsules increased, indicating the successful preparation. The results of enzyme activity test showed that the nanocapsules could not only retain the catalytic activity of AChE, but also greatly improved its high temperature stability and organic solvent stability.

Translated title of the contribution	Synthesis and Stability of Acetylcholinesterase Nanocapsules
Original language	Chinese (Traditional)
Pages (from-to)	246-252
Number of pages	7
Journal	Huaxue Fanying Gongcheng Yu Gongyi/Chemical Reaction Engineering and Technology
Volume	36
Issue number	3
DOIs	https://doi.org/10.11730/j.issn.1001-7631.2020.04.0246.07
Publication status	Published - 25 Jun 2020

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title = "乙酰胆碱酯酶纳米胶囊的合成与稳定性研究",

abstract = "Acetylcholinesterase nanocapsules (nAChE) were prepared by two-step method. Firstly, n-acryloyloxysuccinimide was used to introduce double bonds on the surface of AChE. Then, N-(3-aminopropyl) methacrylate and acrylamide were used as monomers, and N,N'-methylene bisacrylamide was used as crosslinking agent to prepare AChE nanocapsules. The results of dynamic light scattering (DLS) showed that the particle size of AChE nanocapsules increased, indicating the successful preparation. The results of enzyme activity test showed that the nanocapsules could not only retain the catalytic activity of AChE, but also greatly improved its high temperature stability and organic solvent stability.",

keywords = "Acetylcholinesterase, In-situ radical polymerization, Nanocapsule, Organic solvent stability, Thermal stability",

author = "Chengzhao Tu and Meiling Lei and Qingping Cheng and Lin Ye",

year = "2020",

month = jun,

day = "25",

doi = "10.11730/j.issn.1001-7631.2020.04.0246.07",

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volume = "36",

pages = "246--252",

journal = "Huaxue Fanying Gongcheng Yu Gongyi/Chemical Reaction Engineering and Technology",

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publisher = "Zhejiang University",

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TY - JOUR

T1 - 乙酰胆碱酯酶纳米胶囊的合成与稳定性研究

AU - Tu, Chengzhao

AU - Lei, Meiling

AU - Cheng, Qingping

AU - Ye, Lin

PY - 2020/6/25

Y1 - 2020/6/25

N2 - Acetylcholinesterase nanocapsules (nAChE) were prepared by two-step method. Firstly, n-acryloyloxysuccinimide was used to introduce double bonds on the surface of AChE. Then, N-(3-aminopropyl) methacrylate and acrylamide were used as monomers, and N,N'-methylene bisacrylamide was used as crosslinking agent to prepare AChE nanocapsules. The results of dynamic light scattering (DLS) showed that the particle size of AChE nanocapsules increased, indicating the successful preparation. The results of enzyme activity test showed that the nanocapsules could not only retain the catalytic activity of AChE, but also greatly improved its high temperature stability and organic solvent stability.

AB - Acetylcholinesterase nanocapsules (nAChE) were prepared by two-step method. Firstly, n-acryloyloxysuccinimide was used to introduce double bonds on the surface of AChE. Then, N-(3-aminopropyl) methacrylate and acrylamide were used as monomers, and N,N'-methylene bisacrylamide was used as crosslinking agent to prepare AChE nanocapsules. The results of dynamic light scattering (DLS) showed that the particle size of AChE nanocapsules increased, indicating the successful preparation. The results of enzyme activity test showed that the nanocapsules could not only retain the catalytic activity of AChE, but also greatly improved its high temperature stability and organic solvent stability.

KW - Acetylcholinesterase

KW - In-situ radical polymerization

KW - Nanocapsule

KW - Organic solvent stability

KW - Thermal stability

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U2 - 10.11730/j.issn.1001-7631.2020.04.0246.07

DO - 10.11730/j.issn.1001-7631.2020.04.0246.07

M3 - 文章

AN - SCOPUS:85105474137

SN - 1001-7631

VL - 36

SP - 246

EP - 252

JO - Huaxue Fanying Gongcheng Yu Gongyi/Chemical Reaction Engineering and Technology

JF - Huaxue Fanying Gongcheng Yu Gongyi/Chemical Reaction Engineering and Technology

IS - 3

ER -

乙酰胆碱酯酶纳米胶囊的合成与稳定性研究

Abstract

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