α-Chymotrypsin Immobilization on the Surface of Phase-transferred Fe3O4 Nanoparticles

Xia Wu; Yu Shun Han; Min Hua Cao; Chang Wen Hu; Ling Ren; Guang Lu Ge

α-Chymotrypsin Immobilization on the Surface of Phase-transferred Fe₃O₄ Nanoparticles

Xia Wu, Yu Shun Han, Min Hua Cao, Chang Wen Hu, Ling Ren, Guang Lu Ge^*

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The oil-soluble magnetic Fe₃O₄ nanoparticles with uniform size and morphology were synthesized via thermal decomposition of Fe(acac)₃ precursor. They were successfully transferred into aqueous phase through the ligand-exchange reaction with water-soluble PEG diacid molecule, forming water-soluble, stable, nontoxic, and magnetic composite with surface functional group. TEM, M-H curve, FTIR and TGA were used to characterize the as-synthesized composite and demonstrated that the nanoparticles maintained their superparamagnetism and biocompatibility. α-Chymotrypsin(α-ChT) was immobilized on the surface of the water-soluble nanoparticles. The immobilized α-ChT exhibited better thermal stability and high activity after repeated usage.

Original language	English
Pages (from-to)	11-15
Number of pages	5
Journal	Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities
Volume	31
Issue number	1
Publication status	Published - Jan 2010

Keywords

Immobilized enzyme
Magnetic FeO nanoparticle
Surface ligand exchange

Cite this

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title = "α-Chymotrypsin Immobilization on the Surface of Phase-transferred Fe3O4 Nanoparticles",

abstract = "The oil-soluble magnetic Fe3O4 nanoparticles with uniform size and morphology were synthesized via thermal decomposition of Fe(acac)3 precursor. They were successfully transferred into aqueous phase through the ligand-exchange reaction with water-soluble PEG diacid molecule, forming water-soluble, stable, nontoxic, and magnetic composite with surface functional group. TEM, M-H curve, FTIR and TGA were used to characterize the as-synthesized composite and demonstrated that the nanoparticles maintained their superparamagnetism and biocompatibility. α-Chymotrypsin(α-ChT) was immobilized on the surface of the water-soluble nanoparticles. The immobilized α-ChT exhibited better thermal stability and high activity after repeated usage.",

keywords = "Immobilized enzyme, Magnetic FeO nanoparticle, Surface ligand exchange",

author = "Xia Wu and Han, {Yu Shun} and Cao, {Min Hua} and Hu, {Chang Wen} and Ling Ren and Ge, {Guang Lu}",

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language = "English",

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journal = "Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities",

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TY - JOUR

T1 - α-Chymotrypsin Immobilization on the Surface of Phase-transferred Fe3O4 Nanoparticles

AU - Wu, Xia

AU - Han, Yu Shun

AU - Cao, Min Hua

AU - Hu, Chang Wen

AU - Ren, Ling

AU - Ge, Guang Lu

PY - 2010/1

Y1 - 2010/1

N2 - The oil-soluble magnetic Fe3O4 nanoparticles with uniform size and morphology were synthesized via thermal decomposition of Fe(acac)3 precursor. They were successfully transferred into aqueous phase through the ligand-exchange reaction with water-soluble PEG diacid molecule, forming water-soluble, stable, nontoxic, and magnetic composite with surface functional group. TEM, M-H curve, FTIR and TGA were used to characterize the as-synthesized composite and demonstrated that the nanoparticles maintained their superparamagnetism and biocompatibility. α-Chymotrypsin(α-ChT) was immobilized on the surface of the water-soluble nanoparticles. The immobilized α-ChT exhibited better thermal stability and high activity after repeated usage.

AB - The oil-soluble magnetic Fe3O4 nanoparticles with uniform size and morphology were synthesized via thermal decomposition of Fe(acac)3 precursor. They were successfully transferred into aqueous phase through the ligand-exchange reaction with water-soluble PEG diacid molecule, forming water-soluble, stable, nontoxic, and magnetic composite with surface functional group. TEM, M-H curve, FTIR and TGA were used to characterize the as-synthesized composite and demonstrated that the nanoparticles maintained their superparamagnetism and biocompatibility. α-Chymotrypsin(α-ChT) was immobilized on the surface of the water-soluble nanoparticles. The immobilized α-ChT exhibited better thermal stability and high activity after repeated usage.

KW - Immobilized enzyme

KW - Magnetic FeO nanoparticle

KW - Surface ligand exchange

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M3 - Article

AN - SCOPUS:77951453671

SN - 0251-0790

VL - 31

SP - 11

EP - 15

JO - Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities

JF - Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities

IS - 1

ER -

α-Chymotrypsin Immobilization on the Surface of Phase-transferred Fe₃O₄ Nanoparticles

Abstract

Keywords

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