摘要
The binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin (TMPyP4) and the telomeric sequence DNA 5'-TTAGGG-3' (S6) has been explored by means of circular dichroism spectrum, steady-state absorption, steady-state fluorescence and picosecond time-resolved fluorescence spectrums. DNA adopts the single-strand conformation in Tris-HCl buffer without any metal ions. The binding constant and the saturated binding number between TMPyP4 and S6 DNA were determined as 1.51 × 106 (mol/L)-1 and 1.2, respectively, according to steady-state absorption spectroscopy. Furthermore, on the findings of time-resolved fluorescence spectroscopic technique, the binding modes can be presumed.
源语言 | 英语 |
---|---|
页(从-至) | 1365-1368+1373 |
期刊 | Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology |
卷 | 30 |
期 | 11 |
出版状态 | 已出版 - 11月 2010 |