Binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin TMPyP4 with the single-strand DNA 5'-TTAGGG-3'

Hui Juan Zhang*, Xu Kun Chen, Si Ping Pang

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

摘要

The binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin (TMPyP4) and the telomeric sequence DNA 5'-TTAGGG-3' (S6) has been explored by means of circular dichroism spectrum, steady-state absorption, steady-state fluorescence and picosecond time-resolved fluorescence spectrums. DNA adopts the single-strand conformation in Tris-HCl buffer without any metal ions. The binding constant and the saturated binding number between TMPyP4 and S6 DNA were determined as 1.51 × 106 (mol/L)-1 and 1.2, respectively, according to steady-state absorption spectroscopy. Furthermore, on the findings of time-resolved fluorescence spectroscopic technique, the binding modes can be presumed.

源语言英语
页(从-至)1365-1368+1373
期刊Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
30
11
出版状态已出版 - 11月 2010

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Zhang, H. J., Chen, X. K., & Pang, S. P. (2010). Binding mechanism of meso-tetrakis (4-(N-methylpyridiumyl)) porphyrin TMPyP4 with the single-strand DNA 5'-TTAGGG-3'. Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology, 30(11), 1365-1368+1373.