骨肽对骨质疏松大鼠的保护作用

To study the protective effect of bone peptide on osteoporosis induced by retinoic acid in rats, an osteoporosis model was established by orally administration to SD rats with 70 mg/kg retinoic acid for two weeks. After 4-week oral administration of 40 mg/kg etedronate disodium or three doses of bone peptide (1.25, 2.50 and 5.00 g/kg) respectively, the rat femurs were collected for determination of HE, bone density, dry and ash weight, contents of Ca and P. Serum and urine were collected for assay of biochemical indicators. Results show that, compared with the control group, the bone density and bone trabeculae density are decreased in the model group, and the index is significantly increased after oral administration of medium and high doses of bone peptide. Three doses of bone peptide can significantly increase dry and ash weight of femur, contents of Ca and P. They also can increase the contents of BALP and BGP in rat serum, and decrease the level of TRACP-5b. The content of HOP in urine of rats in model group was increased dramatically and decreased significantly after oral administration of low dose of bone peptide. The positive drug showed obvious therapeutic effect in each index. Different doses of bone peptide could show a dose-dependent protective effect, while 2.50 g/kg bone peptide has the most obviously therapeutic effect on osteoporosis induced by retinoic acid in rats.