Upconversion nanoparticle platform for efficient dendritic cell antigen delivery and simultaneous tracking

Zhenfeng Yu; Olena Vepris; Christina Eich; Yansong Feng; Ivo Que; Marcel G.M. Camps; Hong Zhang; Ferry A. Ossendorp; Luis J. Cruz

doi:10.1007/s00604-022-05441-z

Upconversion nanoparticle platform for efficient dendritic cell antigen delivery and simultaneous tracking

Zhenfeng Yu, Olena Vepris, Christina Eich^*, Yansong Feng, Ivo Que, Marcel G.M. Camps, Hong Zhang, Ferry A. Ossendorp, Luis J. Cruz^*

^*Corresponding author for this work

School of Mechatronical Engineering

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Upconversion nanoparticles (UCNPs) represent a group of NPs that can convert near-infrared (NIR) light into ultraviolet and visible light, thus possess deep tissue penetration power with less background fluorescence noise interference, and do not induce damage to biological tissues. Due to their unique optical properties and possibility for surface modification, UCNPs can be exploited for concomitant antigen delivery into dendritic cells (DCs) and monitoring by molecular imaging. In this study, we focus on the development of a nano-delivery platform targeting DCs for immunotherapy and simultaneous imaging. OVA 254–267 (OVA24) peptide antigen, harboring a CD8 T cell epitope, and Pam3CysSerLys4 (Pam3CSK4) adjuvant were chemically linked to the surface of UCNPs by amide condensation to stimulate DC maturation and antigen presentation. The OVA24-Pam3CSK4-UCNPs were thoroughly characterized and showed a homogeneous morphology and surface electronegativity, which promoted a good dispersion of the NPs. In vitro experiments demonstrated that OVA24-Pam3CSK4-UCNPs induced a strong immune response, including DC maturation, T cell activation, and proliferation, as well as interferon gamma (IFN-γ) production. In vivo, highly sensitive upconversion luminescence (UCL) imaging of OVA24-Pam3CSK4-UCNPs allowed tracking of UCNPs from the periphery to lymph nodes. In summary, OVA24-Pam3CSK4-UCNPs represent an effective tool for DC-based immunotherapy. Graphical abstract: [Figure not available: see fulltext.]

Original language	English
Article number	368
Journal	Microchimica Acta
Volume	189
Issue number	10
DOIs	https://doi.org/10.1007/s00604-022-05441-z
Publication status	Published - Oct 2022

Keywords

Dendritic cells
Immunotherapy
In vivo monitoring
OVA
Pam3CSK4
Upconversion nanoparticles

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10.1007/s00604-022-05441-z

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@article{b30ac083fb304a68aa226abff8c9d567,

title = "Upconversion nanoparticle platform for efficient dendritic cell antigen delivery and simultaneous tracking",

abstract = "Upconversion nanoparticles (UCNPs) represent a group of NPs that can convert near-infrared (NIR) light into ultraviolet and visible light, thus possess deep tissue penetration power with less background fluorescence noise interference, and do not induce damage to biological tissues. Due to their unique optical properties and possibility for surface modification, UCNPs can be exploited for concomitant antigen delivery into dendritic cells (DCs) and monitoring by molecular imaging. In this study, we focus on the development of a nano-delivery platform targeting DCs for immunotherapy and simultaneous imaging. OVA 254–267 (OVA24) peptide antigen, harboring a CD8 T cell epitope, and Pam3CysSerLys4 (Pam3CSK4) adjuvant were chemically linked to the surface of UCNPs by amide condensation to stimulate DC maturation and antigen presentation. The OVA24-Pam3CSK4-UCNPs were thoroughly characterized and showed a homogeneous morphology and surface electronegativity, which promoted a good dispersion of the NPs. In vitro experiments demonstrated that OVA24-Pam3CSK4-UCNPs induced a strong immune response, including DC maturation, T cell activation, and proliferation, as well as interferon gamma (IFN-γ) production. In vivo, highly sensitive upconversion luminescence (UCL) imaging of OVA24-Pam3CSK4-UCNPs allowed tracking of UCNPs from the periphery to lymph nodes. In summary, OVA24-Pam3CSK4-UCNPs represent an effective tool for DC-based immunotherapy. Graphical abstract: [Figure not available: see fulltext.]",

keywords = "Dendritic cells, Immunotherapy, In vivo monitoring, OVA, Pam3CSK4, Upconversion nanoparticles",

author = "Zhenfeng Yu and Olena Vepris and Christina Eich and Yansong Feng and Ivo Que and Camps, {Marcel G.M.} and Hong Zhang and Ossendorp, {Ferry A.} and Cruz, {Luis J.}",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = oct,

doi = "10.1007/s00604-022-05441-z",

language = "English",

volume = "189",

journal = "Microchimica Acta",

issn = "0026-3672",

publisher = "Springer-Verlag Wien",

number = "10",

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TY - JOUR

T1 - Upconversion nanoparticle platform for efficient dendritic cell antigen delivery and simultaneous tracking

AU - Yu, Zhenfeng

AU - Vepris, Olena

AU - Eich, Christina

AU - Feng, Yansong

AU - Que, Ivo

AU - Camps, Marcel G.M.

AU - Zhang, Hong

AU - Ossendorp, Ferry A.

AU - Cruz, Luis J.

PY - 2022/10

Y1 - 2022/10

N2 - Upconversion nanoparticles (UCNPs) represent a group of NPs that can convert near-infrared (NIR) light into ultraviolet and visible light, thus possess deep tissue penetration power with less background fluorescence noise interference, and do not induce damage to biological tissues. Due to their unique optical properties and possibility for surface modification, UCNPs can be exploited for concomitant antigen delivery into dendritic cells (DCs) and monitoring by molecular imaging. In this study, we focus on the development of a nano-delivery platform targeting DCs for immunotherapy and simultaneous imaging. OVA 254–267 (OVA24) peptide antigen, harboring a CD8 T cell epitope, and Pam3CysSerLys4 (Pam3CSK4) adjuvant were chemically linked to the surface of UCNPs by amide condensation to stimulate DC maturation and antigen presentation. The OVA24-Pam3CSK4-UCNPs were thoroughly characterized and showed a homogeneous morphology and surface electronegativity, which promoted a good dispersion of the NPs. In vitro experiments demonstrated that OVA24-Pam3CSK4-UCNPs induced a strong immune response, including DC maturation, T cell activation, and proliferation, as well as interferon gamma (IFN-γ) production. In vivo, highly sensitive upconversion luminescence (UCL) imaging of OVA24-Pam3CSK4-UCNPs allowed tracking of UCNPs from the periphery to lymph nodes. In summary, OVA24-Pam3CSK4-UCNPs represent an effective tool for DC-based immunotherapy. Graphical abstract: [Figure not available: see fulltext.]

AB - Upconversion nanoparticles (UCNPs) represent a group of NPs that can convert near-infrared (NIR) light into ultraviolet and visible light, thus possess deep tissue penetration power with less background fluorescence noise interference, and do not induce damage to biological tissues. Due to their unique optical properties and possibility for surface modification, UCNPs can be exploited for concomitant antigen delivery into dendritic cells (DCs) and monitoring by molecular imaging. In this study, we focus on the development of a nano-delivery platform targeting DCs for immunotherapy and simultaneous imaging. OVA 254–267 (OVA24) peptide antigen, harboring a CD8 T cell epitope, and Pam3CysSerLys4 (Pam3CSK4) adjuvant were chemically linked to the surface of UCNPs by amide condensation to stimulate DC maturation and antigen presentation. The OVA24-Pam3CSK4-UCNPs were thoroughly characterized and showed a homogeneous morphology and surface electronegativity, which promoted a good dispersion of the NPs. In vitro experiments demonstrated that OVA24-Pam3CSK4-UCNPs induced a strong immune response, including DC maturation, T cell activation, and proliferation, as well as interferon gamma (IFN-γ) production. In vivo, highly sensitive upconversion luminescence (UCL) imaging of OVA24-Pam3CSK4-UCNPs allowed tracking of UCNPs from the periphery to lymph nodes. In summary, OVA24-Pam3CSK4-UCNPs represent an effective tool for DC-based immunotherapy. Graphical abstract: [Figure not available: see fulltext.]

KW - Dendritic cells

KW - Immunotherapy

KW - In vivo monitoring

KW - OVA

KW - Pam3CSK4

KW - Upconversion nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=85137163106&partnerID=8YFLogxK

U2 - 10.1007/s00604-022-05441-z

DO - 10.1007/s00604-022-05441-z

M3 - Article

C2 - 36057018

AN - SCOPUS:85137163106

SN - 0026-3672

VL - 189

JO - Microchimica Acta

JF - Microchimica Acta

IS - 10

M1 - 368

ER -

Upconversion nanoparticle platform for efficient dendritic cell antigen delivery and simultaneous tracking

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Keywords

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