T1-Mediated Nanosensor for Immunoassay Based on an Activatable MnO2 Nanoassembly

Zixin Liu; Yunlei Xianyu; Wenshu Zheng; Jiangjiang Zhang; Yunjing Luo; Yiping Chen; Mingling Dong; Jing Wu; Xingyu Jiang

doi:10.1021/acs.analchem.7b04817

T₁-Mediated Nanosensor for Immunoassay Based on an Activatable MnO₂ Nanoassembly

Zixin Liu, Yunlei Xianyu, Wenshu Zheng, Jiangjiang Zhang, Yunjing Luo^*, Yiping Chen, Mingling Dong, Jing Wu, Xingyu Jiang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Current magnetic relaxation switching (MRS) sensors for detection of trace targets in complex samples still suffer from limitations in terms of relatively low sensitivity and poor stability. To meet this challenge, we develop a longitudinal relaxation time (T₁)-based nanosensor by using Mn²⁺ released from the reduction of a MnO₂ nanoassembly that can induce the change of T₁, and thus can greatly improve the sensitivity and overcome the "hook effect" of conventional MRS. Through the specific interaction between antigen and the antibody-functionalized MnO₂ nanoassembly, the T₁ signal of Mn²⁺ released from the nanoassembly is quantitatively determined by the antigen, which allows for highly sensitive and straightforward detection of targets. This approach broadens the applicability of magnetic biosensors and has great potential for applications in early diagnosis of disease biomarkers.

Original language	English
Pages (from-to)	2765-2771
Number of pages	7
Journal	Analytical Chemistry
Volume	90
Issue number	4
DOIs	https://doi.org/10.1021/acs.analchem.7b04817
Publication status	Published - 20 Feb 2018
Externally published	Yes

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title = "T1-Mediated Nanosensor for Immunoassay Based on an Activatable MnO2 Nanoassembly",

abstract = "Current magnetic relaxation switching (MRS) sensors for detection of trace targets in complex samples still suffer from limitations in terms of relatively low sensitivity and poor stability. To meet this challenge, we develop a longitudinal relaxation time (T1)-based nanosensor by using Mn2+ released from the reduction of a MnO2 nanoassembly that can induce the change of T1, and thus can greatly improve the sensitivity and overcome the {"}hook effect{"} of conventional MRS. Through the specific interaction between antigen and the antibody-functionalized MnO2 nanoassembly, the T1 signal of Mn2+ released from the nanoassembly is quantitatively determined by the antigen, which allows for highly sensitive and straightforward detection of targets. This approach broadens the applicability of magnetic biosensors and has great potential for applications in early diagnosis of disease biomarkers.",

author = "Zixin Liu and Yunlei Xianyu and Wenshu Zheng and Jiangjiang Zhang and Yunjing Luo and Yiping Chen and Mingling Dong and Jing Wu and Xingyu Jiang",

note = "Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

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doi = "10.1021/acs.analchem.7b04817",

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T1 - T1-Mediated Nanosensor for Immunoassay Based on an Activatable MnO2 Nanoassembly

AU - Liu, Zixin

AU - Xianyu, Yunlei

AU - Zheng, Wenshu

AU - Zhang, Jiangjiang

AU - Luo, Yunjing

AU - Chen, Yiping

AU - Dong, Mingling

AU - Wu, Jing

AU - Jiang, Xingyu

PY - 2018/2/20

Y1 - 2018/2/20

N2 - Current magnetic relaxation switching (MRS) sensors for detection of trace targets in complex samples still suffer from limitations in terms of relatively low sensitivity and poor stability. To meet this challenge, we develop a longitudinal relaxation time (T1)-based nanosensor by using Mn2+ released from the reduction of a MnO2 nanoassembly that can induce the change of T1, and thus can greatly improve the sensitivity and overcome the "hook effect" of conventional MRS. Through the specific interaction between antigen and the antibody-functionalized MnO2 nanoassembly, the T1 signal of Mn2+ released from the nanoassembly is quantitatively determined by the antigen, which allows for highly sensitive and straightforward detection of targets. This approach broadens the applicability of magnetic biosensors and has great potential for applications in early diagnosis of disease biomarkers.

AB - Current magnetic relaxation switching (MRS) sensors for detection of trace targets in complex samples still suffer from limitations in terms of relatively low sensitivity and poor stability. To meet this challenge, we develop a longitudinal relaxation time (T1)-based nanosensor by using Mn2+ released from the reduction of a MnO2 nanoassembly that can induce the change of T1, and thus can greatly improve the sensitivity and overcome the "hook effect" of conventional MRS. Through the specific interaction between antigen and the antibody-functionalized MnO2 nanoassembly, the T1 signal of Mn2+ released from the nanoassembly is quantitatively determined by the antigen, which allows for highly sensitive and straightforward detection of targets. This approach broadens the applicability of magnetic biosensors and has great potential for applications in early diagnosis of disease biomarkers.

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U2 - 10.1021/acs.analchem.7b04817

DO - 10.1021/acs.analchem.7b04817

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EP - 2771

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 4

ER -

T₁-Mediated Nanosensor for Immunoassay Based on an Activatable MnO₂ Nanoassembly

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