The synthesis and characterization of β-cyclodextrin derivated pancreatin used as chiral selector in capillary electrochromatography

Rongji Dai*, Li Tang, Bin Tong, Yulin Deng, Ruonong Fu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The preparation of capillary electrochromatography column for chiral separation with β-cyclo-dextrin derivated pancreatin was investigated, which had three steps, synthesis of β-cyclodextrin derivated pancreatin, the modification of the capillary internal surface and the covalent binding of β-cyclodextrin derivated pancreatin on the capillary internal wall. β-cyclodextrin derivated pancreatin was synthesized using β-cyclodextrin and protein in the presence of ethylene glycol diglycidyl ether (EGDE) in boric acid buffer at pH = 8.7. Amino group was coated on the internal surface of the silica capillary by sol-gel technology using triethoxylmethylsiloxane and (3-aminopropyl) trimethoxysiloxane. Covalent binding of β-cyclodextrin derivated pancreatin was performed by glutaraldehyde. Chlorpheniramine, phenylalanine and troopine were separated baselines by β-cyclodextrin derivated pancreatin in capillary electrochromatography.

Original languageEnglish
Pages (from-to)27-28
Number of pages2
JournalJournal Wuhan University of Technology, Materials Science Edition
Volume20
Issue numberSUPPL.
Publication statusPublished - Dec 2005

Keywords

  • Capillary electrochromatography
  • Chiral separation
  • Pancreatin
  • β-cyclodextrin

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