Abstract
The preparation of capillary electrochromatography column for chiral separation with β-cyclo-dextrin derivated pancreatin was investigated, which had three steps, synthesis of β-cyclodextrin derivated pancreatin, the modification of the capillary internal surface and the covalent binding of β-cyclodextrin derivated pancreatin on the capillary internal wall. β-cyclodextrin derivated pancreatin was synthesized using β-cyclodextrin and protein in the presence of ethylene glycol diglycidyl ether (EGDE) in boric acid buffer at pH = 8.7. Amino group was coated on the internal surface of the silica capillary by sol-gel technology using triethoxylmethylsiloxane and (3-aminopropyl) trimethoxysiloxane. Covalent binding of β-cyclodextrin derivated pancreatin was performed by glutaraldehyde. Chlorpheniramine, phenylalanine and troopine were separated baselines by β-cyclodextrin derivated pancreatin in capillary electrochromatography.
Original language | English |
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Pages (from-to) | 27-28 |
Number of pages | 2 |
Journal | Journal Wuhan University of Technology, Materials Science Edition |
Volume | 20 |
Issue number | SUPPL. |
Publication status | Published - Dec 2005 |
Keywords
- Capillary electrochromatography
- Chiral separation
- Pancreatin
- β-cyclodextrin