Super-resolution nanoscopy by coherent control on nanoparticle emission

Congyue Liu; Wei Liu; Shufeng Wang; Hongjia Li; Zhilong Lv; Fa Zhang; Donghui Zhang; Junlin Teng; Tao Zheng; Donghai Li; Mingshu Zhang; Pingyong Xu; Qihuang Gong

doi:10.1126/sciadv.aaw6579

Super-resolution nanoscopy by coherent control on nanoparticle emission

Congyue Liu, Wei Liu, Shufeng Wang, Hongjia Li, Zhilong Lv, Fa Zhang, Donghui Zhang, Junlin Teng, Tao Zheng, Donghai Li, Mingshu Zhang, Pingyong Xu, Qihuang Gong^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Super-resolution nanoscopy based on wide-field microscopic imaging provided high efficiency but limited resolution. Here, we demonstrate a general strategy to push its resolution down to ∼50 nm, which is close to the range of single molecular localization microscopy, without sacrificing the wide-field imaging advantage. It is done by actively and simultaneously modulating the characteristic emission of each individual emitter at high density. This method is based on the principle of excited state coherent control on single-particle two-photon fluorescence. In addition, the modulation efficiently suppresses the noise for imaging. The capability of the method is verified both in simulation and in experiments on ZnCdS quantum dot-labeled films and COS7 cells. The principle of coherent control is generally applicable to single-multiphoton imaging and various probes.

Original language	English
Article number	eaaw6579
Journal	Science advances
Volume	6
Issue number	16
DOIs	https://doi.org/10.1126/sciadv.aaw6579
Publication status	Published - Apr 2020
Externally published	Yes

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title = "Super-resolution nanoscopy by coherent control on nanoparticle emission",

abstract = "Super-resolution nanoscopy based on wide-field microscopic imaging provided high efficiency but limited resolution. Here, we demonstrate a general strategy to push its resolution down to ∼50 nm, which is close to the range of single molecular localization microscopy, without sacrificing the wide-field imaging advantage. It is done by actively and simultaneously modulating the characteristic emission of each individual emitter at high density. This method is based on the principle of excited state coherent control on single-particle two-photon fluorescence. In addition, the modulation efficiently suppresses the noise for imaging. The capability of the method is verified both in simulation and in experiments on ZnCdS quantum dot-labeled films and COS7 cells. The principle of coherent control is generally applicable to single-multiphoton imaging and various probes.",

author = "Congyue Liu and Wei Liu and Shufeng Wang and Hongjia Li and Zhilong Lv and Fa Zhang and Donghui Zhang and Junlin Teng and Tao Zheng and Donghai Li and Mingshu Zhang and Pingyong Xu and Qihuang Gong",

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AU - Liu, Congyue

AU - Liu, Wei

AU - Wang, Shufeng

AU - Li, Hongjia

AU - Lv, Zhilong

AU - Zhang, Fa

AU - Zhang, Donghui

AU - Teng, Junlin

AU - Zheng, Tao

AU - Li, Donghai

AU - Zhang, Mingshu

AU - Xu, Pingyong

AU - Gong, Qihuang

PY - 2020/4

Y1 - 2020/4

N2 - Super-resolution nanoscopy based on wide-field microscopic imaging provided high efficiency but limited resolution. Here, we demonstrate a general strategy to push its resolution down to ∼50 nm, which is close to the range of single molecular localization microscopy, without sacrificing the wide-field imaging advantage. It is done by actively and simultaneously modulating the characteristic emission of each individual emitter at high density. This method is based on the principle of excited state coherent control on single-particle two-photon fluorescence. In addition, the modulation efficiently suppresses the noise for imaging. The capability of the method is verified both in simulation and in experiments on ZnCdS quantum dot-labeled films and COS7 cells. The principle of coherent control is generally applicable to single-multiphoton imaging and various probes.

AB - Super-resolution nanoscopy based on wide-field microscopic imaging provided high efficiency but limited resolution. Here, we demonstrate a general strategy to push its resolution down to ∼50 nm, which is close to the range of single molecular localization microscopy, without sacrificing the wide-field imaging advantage. It is done by actively and simultaneously modulating the characteristic emission of each individual emitter at high density. This method is based on the principle of excited state coherent control on single-particle two-photon fluorescence. In addition, the modulation efficiently suppresses the noise for imaging. The capability of the method is verified both in simulation and in experiments on ZnCdS quantum dot-labeled films and COS7 cells. The principle of coherent control is generally applicable to single-multiphoton imaging and various probes.

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Super-resolution nanoscopy by coherent control on nanoparticle emission

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